ArticleBiophysical journal2002
Exocytosis in bovine chromaffin cells: studies with patch-clamp capacitance and FM1-43 fluorescence.
Article in Biophysical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 20 citations in OpenAlex.
- 5 ns electric pulses induce CaBioelectrochemistry (Amsterdam, Netherlands) · 2021Article
- Article
- Evidence for sustained ATP release from liver cells that is not mediated by vesicular exocytosis.Purinergic signalling · 2011Article
- 5-Nitro-2-(3-phenylpropylamino)benzoic acid (NPPB) stimulates cellular ATP release through exocytosis of ATP-enriched vesicles.The Journal of biological chemistry · 2009Article
- Distinctive roles of different beta-amyloid 42 aggregates in modulation of synaptic functions.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2009Article
- Hypotonicity and peptide discharge from a single vesicle.American journal of physiology. Cell physiology · 2008Article
- Massive Ca-induced membrane fusion and phospholipid changes triggered by reverse Na/Ca exchange in BHK fibroblasts.The Journal of general physiology · 2008Article
- Evidence for AMPK-dependent regulation of exocytosis of lipoproteins in a model liver cell line.Experimental cell research · 2008Article
- Effects of hyposmotic stress on exocytosis in isolated turbot, Scophthalmus maximus, hepatocytes.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2006Article
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In response to physiological stimuli, neuroendocrine cells secrete neurotransmitters through a Ca(2+)-dependent fusion of secretory granules with the plasma membrane. We studied insertion of granules in bovine chromaffin cells using capacitance as a measure of plasma membrane area and fluorescence of a membrane marker FM1-43 as a measure of exocytosis. Intracellular dialysis with [Ca(2+)] (1.5-100 microM) evoked massive exocytosis that was sufficient to double plasma membrane area but did not swell cells. In principle, in the absence of endocytosis, the addition of granule membrane would be anticipated to produce similar increases in the capacitance and FM1-43 fluorescence responses. However, when endocytosis was minimal, the changes in capacitance were markedly larger than the corresponding changes in FM1-43 fluorescence. Moreover, the apparent differences between capacitance and FM1-43 fluorescence changes increased with larger exocytic responses, as more granules fused with the plasma membrane. In experiments in which exocytosis was suppressed, increasing membrane tension by osmotically induced cell swelling increased FM1-43 fluorescence, suggesting that FM1-43 fluorescence is sensitive to changes in the membrane tension. Thus, increasing membrane area through exocytosis does not swell chromaffin cells but may decrease membrane tension.
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