ArticleBiophysical journal2002
Fluid shear regulates the kinetics and molecular mechanisms of activation-dependent platelet binding to colon carcinoma cells.
Article in Biophysical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed, 64 citations in OpenAlex.
- Fundamentals of Bowel Cancer for Biomedical Engineers.Annals of biomedical engineering · 2023Review
- Review
- Aspirin and antiplatelet treatments in cancer.Blood · 2021Article
- Surgery for Colorectal Cancer: A Trigger for Liver Metastases Development? New Insights into the Underlying Mechanisms.Biomedicines · 2021Review
- Circulatory shear stress induces molecular changes and side population enrichment in primary tumor-derived lung cancer cells with higher metastatic potential.Scientific reports · 2021Article
- Impact of Polypharmacy for Chronic Ailments in Colon Cancer Patients: A Review Focused on Drug Repurposing.Cancers · 2020Review
- Platelet mediated TRAIL delivery for efficiently targeting circulating tumor cells.Nanoscale advances · 2020Article
- Matrix Stiffness and Colorectal Cancer.OncoTargets and therapy · 2020Review
- Hepatic thrombopoietin gene silencing reduces platelet count and breast cancer progression in transgenic MMTV-PyMT mice.Blood advances · 2019Article
- The role of coagulation and platelets in colon cancer-associated thrombosis.American journal of physiology. Cell physiology · 2019Article
- Novel antibodies against GPIbα inhibit pulmonary metastasis by affecting vWF-GPIbα interaction.Journal of hematology & oncology · 2018Article
- E-selectin-mediated rolling facilitates pancreatic cancer cell adhesion to hyaluronic acid.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2017Article
- Platelet count as a predictor of metastasis and venous thromboembolism in patients with cancer.Convergent science physical oncology · 2017Article
- Aspirin therapy reduces the ability of platelets to promote colon and pancreatic cancer cell proliferation: Implications for the oncoprotein c-MYC.American journal of physiology. Cell physiology · 2017Article
- The prothrombotic activity of cancer cells in the circulation.Blood reviews · 2016Review
- A Microfluidic Method for the Selection of Undifferentiated Human Embryonic Stem Cells andMicrofluidics and nanofluidics · 2015Article
- Measurement science in the circulatory system.Cellular and molecular bioengineering · 2014Article
- Modeling tumor microenvironments in vitro.Journal of biomechanical engineering · 2014Review
- Physical biology in cancer. 2. The physical biology of circulating tumor cells.American journal of physiology. Cell physiology · 2014Review
- Role of coagulation in the recruitment of colon adenocarcinoma cells to thrombus under shear.American journal of physiology. Cell physiology · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study was undertaken to investigate the kinetics and molecular requirements of platelet binding to tumor cells in bulk suspensions subjected to a uniform linear shear field, using a human colon adenocarcinoma cell line (LS174T) as a model. The effects of shear rate (20-1000 s(-1)), shear exposure time (30-300 s), shear stress (at constant shear rate by adjusting the viscosity of the medium from 1.3-2.6 cP), cell concentration, and platelet activation on platelet-LS174T heteroaggregation were assessed. The results indicate that hydrodynamic shear-induced collisions augment platelet-LS174T binding, which is further potentiated by thrombin/GPRP-NH(2). Peak adhesion efficiency occurs at low shear and decreases with increasing shear. Intercellular contact duration is the predominant factor limiting heteroaggregation at shear rates up to 200 s(-1), whereas these interactions become shear stress-sensitive at > or = 400 s(-1). Heteroaggregation increases with platelet concentration due to an elevation of the intercellular collision frequency, whereas adhesion efficiency remains nearly constant. Moreover, hydrodynamic shear affects the receptor specificity of activation-dependent platelet binding to LS174T cells, as evidenced by the transition from a P-selectin-independent/Arg-Gly-Asp (RGD)-dependent process at 100 s(-1) to a P-selectin/alpha(IIb)beta(3)-dependent interaction at 800 s(-1). This study demonstrates that platelet activation and a fluid-mechanical environment representative of the vasculature affect platelet-tumor cell adhesive interactions pertinent to the process of blood-borne metastasis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.