ArticleBMC plant biology2002
High frequency of phenotypic deviations in Physcomitrella patens plants transformed with a gene-disruption library.
Article in BMC plant biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 97 citations in OpenAlex.
- Multiplication of peat moss (Sphagnum L.) species for climate action.Journal of experimental botany · 2026Review
- The secreted redox sensor roGFP2-Orp1 reveals oxidative dynamics in the plant apoplast.Plant biology (Stuttgart, Germany) · 2026Article
- Functional characterisation of Target of Rapamycin (TOR) signalling in Physcomitrella.Plant cell reports · 2026Article
- Secretion-based production of prolyl-hydroxylated human type III collagen in scalable Physcomitrella photobioreactors.Plant cell reports · 2026Article
- Recombinant production of spider silk protein in Physcomitrella photobioreactors.Plant cell reports · 2025Article
- Differential prolyl hydroxylation by six Physcomitrella prolyl-4 hydroxylases.Computational and structural biotechnology journal · 2024Article
- Multifactorial analysis of terminator performance on heterologous gene expression in Physcomitrella.Plant cell reports · 2024Article
- Long-Distance Electrical and Calcium Signals Evoked by Hydrogen Peroxide in Physcomitrella.Plant & cell physiology · 2023Article
- A deeply conserved protease, acylamino acid-releasing enzyme (AARE), acts in ageing in Physcomitrella and Arabidopsis.Communications biology · 2023Article
- The advent of plant cells in bioreactors.Frontiers in plant science · 2023Review
- Spindle motility skews division site determination during asymmetric cell division in Physcomitrella.Nature communications · 2022Article
- Autopolyploidization affects transcript patterns and gene targeting frequencies in Physcomitrella.Plant cell reports · 2022Article
- Stomata and Sporophytes of the Model MossFrontiers in plant science · 2020Article
- Physcomitrella patens, a versatile synthetic biology chassis.Plant cell reports · 2018Review
- Article
- Microscopy ofPlant methods · 2017Article
- Origin and function of stomata in the moss Physcomitrella patens.Nature plants · 2016Article
- Identification of Targets and Interaction Partners of Arginyl-tRNA Protein Transferase in the Moss Physcomitrella patens.Molecular & cellular proteomics : MCP · 2016Article
- Moss-made pharmaceuticals: from bench to bedside.Plant biotechnology journal · 2015Review
- HIGH CHLOROPHYLL FLUORESCENCE145 Binds to and Stabilizes the psaA 5' UTR via a Newly Defined Repeat Motif in Embryophyta.The Plant cell · 2015Article
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Authors and funding
14 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe moss Physcomitrella patens is an attractive model system for plant biology and functional genome analysis. It shares many biological features with higher plants but has the unique advantage of an efficient homologous recombination system for its nuclear DNA. This allows precise genetic manipulations and targeted knockouts to study gene function, an approach that due to the very low frequency of targeted recombination events is not routinely possible in any higher plant.
resultsAs an important prerequisite for a large-scale gene/function correlation study in this plant, we are establishing a collection of Physcomitrella patens transformants with insertion mutations in most expressed genes. A low-redundancy moss cDNA library was mutagenised in E. coli using a derivative of the transposon Tn1000. The resulting gene-disruption library was then used to transform Physcomitrella. Homologous recombination of the mutagenised cDNA with genomic coding sequences is expected to target insertion events preferentially to expressed genes. An immediate phenotypic analysis of transformants is made possible by the predominance of the haploid gametophytic state in the life cycle of the moss. Among the first 16,203 transformants analysed so far, we observed 2636 plants (= 16.2%) that differed from the wild-type in a variety of developmental, morphological and physiological characteristics.
conclusionsThe high proportion of phenotypic deviations and the wide range of abnormalities observed among the transformants suggests that mutagenesis by gene-disruption library transformation is a useful strategy to establish a highly diverse population of Physcomitrella patens mutants for functional genome analysis.
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