ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2002
Ischemia induces a translocation of the splicing factor tra2-beta 1 and changes alternative splicing patterns in the brain.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 86 citations in OpenAlex.
- Review
- Calcium signalling in weeds under herbicide stress: An outlook.Frontiers in plant science · 2023Article
- miR-335 inhibited cell proliferation of lung cancer cells by target Tra2β.Cancer science · 2018Article
- Stk38 Modulates Rbm24 Protein Stability to Regulate Sarcomere Assembly in Cardiomyocytes.Scientific reports · 2017Article
- The Musashi 1 Controls the Splicing of Photoreceptor-Specific Exons in the Vertebrate Retina.PLoS genetics · 2016Article
- Intra-domain Cross-talk Regulates Serine-arginine Protein Kinase 1-dependent Phosphorylation and Splicing Function of Transformer 2β1.The Journal of biological chemistry · 2015Article
- HNRNP G and HTRA2-BETA1 regulate estrogen receptor alpha expression with potential impact on endometrial cancer.BMC cancer · 2015Article
- Dynamic changes in DNA methylation in ischemic tolerance.Frontiers in neurology · 2015Article
- Calcium-mediated histone modifications regulate alternative splicing in cardiomyocytes.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Transformer 2β (Tra2β/SFRS10) positively regulates the progression of NSCLC via promoting cell proliferation.Journal of molecular histology · 2014Article
- Emerging mechanisms and consequences of calcium regulation of alternative splicing in neurons and endocrine cells.Cellular and molecular life sciences : CMLS · 2013Review
- Characterization of nuclear localization signals (NLSs) and function of NLSs and phosphorylation of serine residues in subcellular and subnuclear localization of transformer-2β (Tra2β).The Journal of biological chemistry · 2013Article
- Alternative splicing: a pivotal step between eukaryotic transcription and translation.Nature reviews. Molecular cell biology · 2013Review
- Tra2-mediated recognition of HIV-1 5' splice site D3 as a key factor in the processing of vpr mRNA.Journal of virology · 2013Article
- Function of alternative splicing.Gene · 2013Review
- Expression of Tra2 β in Cancer Cells as a Potential Contributory Factor to Neoplasia and Metastasis.International journal of cell biology · 2013Article
- Dynamic expression pattern of neuro-oncological ventral antigen 1 (Nova1) in the rat brain after focal cerebral ischemia/reperfusion insults.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2013Article
- Expression analysis of an evolutionarily conserved alternative splicing factor, Sfrs10, in age-related macular degeneration.PloS one · 2013Article
- SPLOOCE: a new portal for the analysis of human splicing variants.RNA biology · 2012Article
- C6 pyridinium ceramide influences alternative pre-mRNA splicing by inhibiting protein phosphatase-1.Nucleic acids research · 2012Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Alternative splice-site selection is regulated by the relative concentration of individual members of the serine-arginine family of proteins and heterogeneous nuclear ribonucleoproteins. Most of these proteins accumulate predominantly in the nucleus, and a subset of them shuttles continuously between nucleus and cytosol. We demonstrate that in primary neuronal cultures, a rise in intracellular calcium concentration induced by thapsigargin leads to a translocation of the splicing regulatory protein tra2-beta1 and a consequent change in splice-site selection. To investigate this phenomenon under physiological conditions, we used an ischemia model. Ischemia induced in the brain causes a cytoplasmic accumulation and hyperphosphorylation of tra2-beta1. In addition, several of the proteins binding to tra2-beta1, such as src associated in mitosis 68 and serine/arginine-rich proteins, accumulate in the cytosol. Concomitant with this subcellular relocalization, we observed a change in alternative splice-site usage of the ICH-1 gene. The increased usage of its alternative exons is in agreement with previous studies demonstrating its repression by a high concentration of proteins with serine/arginine-rich domains. Our findings suggest that a change in the calcium concentration associated with ischemia is part of a signaling event, which changes pre-mRNA splicing pathways by causing relocalization of proteins that regulate splice-site selection.
Indexed as
Identifiers
12122051PMC6757936W2142998358What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.