ArticleAntimicrobial agents and chemotherapy2002
Stress-based identification and classification of antibacterial agents: second-generation Escherichia coli reporter strains and optimization of detection.
Article in Antimicrobial agents and chemotherapy, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 71 citations in OpenAlex.
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- Chemical genetic approaches for the discovery of bacterial cell wall inhibitors.RSC medicinal chemistry · 2023Review
- Production of Ramoplanin and Ramoplanin Analogs by Actinomycetes.Frontiers in microbiology · 2017Article
- Techniques for Screening Translation Inhibitors.Antibiotics (Basel, Switzerland) · 2016Review
- Evaluation of ciprofloxacin and metronidazole encapsulated biomimetic nanomatrix gel onBiomaterials research · 2015Article
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- A novel high-throughput cell-based assay aimed at identifying inhibitors of DNA metabolism in bacteria.Antimicrobial agents and chemotherapy · 2014Article
- Sequencing and comparative genomics analysis inBiotechnology, biotechnological equipment · 2014Article
- Novel whole-cell Reporter Assay for Stress-Based Classification of Antibacterial Compounds Produced by Locally Isolated Bacillus spp.Indian journal of microbiology · 2012Article
- Attenuation-based dual-fluorescent-protein reporter for screening translation inhibitors.Antimicrobial agents and chemotherapy · 2012Article
- 6-Arylpyrido[2,3-d]pyrimidines as novel ATP-competitive inhibitors of bacterial D-alanine:D-alanine ligase.PloS one · 2012Article
- Activity of and development of resistance to corallopyronin A, an inhibitor of RNA polymerase.Antimicrobial agents and chemotherapy · 2011Article
- The Escherichia coli BtuE protein functions as a resistance determinant against reactive oxygen species.PloS one · 2011Article
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- Biological activities of novel gyrase inhibitors of the aminocoumarin class.Antimicrobial agents and chemotherapy · 2008Article
- Cysteine metabolism-related genes and bacterial resistance to potassium tellurite.Journal of bacteriology · 2007Article
- Novel whole-cell antibiotic biosensors for compound discovery.Applied and environmental microbiology · 2007Article
- Article
- Role for tandem duplication and lon protease in AcrAB-TolC- dependent multiple antibiotic resistance (Mar) in an Escherichia coli mutant without mutations in marRAB or acrRAB.Journal of bacteriology · 2006Article
- Construction of a ColD cda promoter-based SOS-green fluorescent protein whole-cell biosensor with higher sensitivity toward genotoxic compounds than constructs based on recA, umuDC, or sulA promoters.Applied and environmental microbiology · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Escherichia coli strains bearing single-copy fusions between the lacZ reporter gene and the cspA, ibp, or P3rpoH stress promoters offer a simple means to detect sublethal concentrations of antibacterial agents interfering with prokaryotic translation or cell envelope integrity while simultaneously providing information on the mechanism of action of the test compound (A. A. Bianchi and F. Baneyx, Appl. Environ. Microbiol. 65:5023-5027, 1999). Here, we expand the usefulness of this system by (i) demonstrating that a fusion between the SOS-inducible sulA promoter and lacZ is a highly specific probe for the detection of antimicrobial agents that ultimately interfere with DNA replication, (ii) showing that inactivation of the tolC gene allows efficient detection of very low concentrations of model antibiotics (including aminoglycosides) whereas polymyxin B-mediated outer membrane permeabilization facilitates the identification of intermediate concentrations of hydrophobic compounds, and (iii) validating the potential of detector strains and sensitization strategies for high-throughput screening using a reproducible and internally consistent 96-well microplate assay.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.