ArticleAntimicrobial agents and chemotherapy2002
Analysis of penicillin-binding protein genes of clinical isolates of Streptococcus pneumoniae with reduced susceptibility to amoxicillin.
Article in Antimicrobial agents and chemotherapy, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 78 citations in OpenAlex.
- Evolution and rise of antimicrobial resistance in key public health bacteria within and across the One Health sectors.Future microbiology · 2026Review
- The effect of MurM and a branched cell wall structure on penicillin resistance inJournal of bacteriology · 2025Article
- Seven draft genome sequences ofMicrobiology resource announcements · 2024Article
- The acquisition of clinically relevant amoxicillin resistance in Streptococcus pneumoniae requires ordered horizontal gene transfer of four loci.PLoS pathogens · 2022Article
- Molecular Characterization of Penicillin-Binding Protein2x, 2b and 1a ofFrontiers in microbiology · 2022Article
- New Insights into Beta-Lactam Resistance ofMicroorganisms · 2021Article
- The role of interspecies recombination in the evolution of antibiotic-resistant pneumococci.eLife · 2021Article
- Antimicrobial Resistance Profiles and Genes inFrontiers in veterinary science · 2021Article
- Genomic epidemiology of penicillin-non-susceptibleMicrobial genomics · 2019Review
- Antimicrobial resistance, penicillin-binding protein sequences, and pilus islet carriage in relation to clonal evolution of Streptococcus pneumoniae serotype 19A in Russia, 2002-2013.Epidemiology and infection · 2017Article
- Molecular characteristics of penicillin-binding protein 2b, 2x and 1a sequences in Streptococcus pneumoniae isolates causing invasive diseases among children in Northeast China.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2016Article
- Molecular analysis of pbp2b in Streptococcus pneumonia isolated from clinical and normal flora samples.Current microbiology · 2015Article
- Commensal streptococci serve as a reservoir for β-lactam resistance genes in Streptococcus pneumoniae.Antimicrobial agents and chemotherapy · 2015Article
- Spread of Streptococcus pneumoniae serotype 8-ST63 multidrug-resistant recombinant Clone, Spain.Emerging infectious diseases · 2014Article
- Genomic analyses of DNA transformation and penicillin resistance in Streptococcus pneumoniae clinical isolates.Antimicrobial agents and chemotherapy · 2014Article
- Whole genome sequencing of penicillin-resistant Streptococcus pneumoniae reveals mutations in penicillin-binding proteins and in a putative iron permease.Genome biology · 2011Article
- Expansion and evolution of the Streptococcus pneumoniae Spain9V-ST156 clonal complex in Poland.Antimicrobial agents and chemotherapy · 2010Article
- Comparative antipneumococcal activities of sulopenem and other drugs.Antimicrobial agents and chemotherapy · 2009Article
- Influences of capsule on cell shape and chain formation of wild-type and pcsB mutants of serotype 2 Streptococcus pneumoniae.Journal of bacteriology · 2009Article
- An important site in PBP2x of penicillin-resistant clinical isolates of Streptococcus pneumoniae: mutational analysis of Thr338.Antimicrobial agents and chemotherapy · 2009Article
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Authors and funding
3 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The recent emergence of pneumococcal isolates exhibiting an unusual resistance phenotype of higher amoxicillin MICs in relation to the penicillin MICs prompted an analysis of the pbp genes from three such strains isolated in France. For comparison, three amoxicillin-susceptible strains were included in the study. DNA sequence analysis of the pbp2x, pbp2b, and pbp1a genes revealed extensive sequence divergence in all six isolates compared to the sequences of the genes of penicillin-susceptible strain R6. With the exception of pbp2b, no amino acid mutations were unique to the resistant isolates. Transformation experiments with cloned pbp genes isolated from one of the resistant isolates demonstrated a stepwise development of amoxicillin resistance involving penicillin-binding proteins (PBPs) 2X, 2B, and 1A. Full resistance, equivalent to that of the donor strain, was achieved only when genomic DNA was transformed into R6(2x/2b/1a) mutants, suggesting that full resistance development in this isolate is mediated by a non-PBP determinant. Moreover, the recently identified murMN resistance determinant does not appear to have any impact on resistance in this isolate. This determinant (from the French isolate) was, however, able to transform an R6 mutant harboring pbp2x, pbp2b, and pbp1a genes from a Hungarian clone with an extremely high level of penicillin resistance so that it had increased levels of penicillin resistance. These results indicate that the development of high-level beta-lactam resistance is a complex process and that the involvement of MurMN in penicillin resistance appears to be dependent on specific mutations in PBPs 2X, 2B, and/or 1A. Furthermore, an additional (as yet unidentified) non-PBP-mediated resistance determinant is required for full resistance development in some pneumococci.
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