ArticleThe Plant cell2002
Detection and localization of a chloroplast-encoded HU-like protein that organizes chloroplast nucleoids.
Article in The Plant cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 84 citations in OpenAlex.
- Article
- Phase separation may drive mitochondrial nucleoid compartmentation.Metabolism open · 2022Article
- Article
- HBD1 protein with a tandem repeat of two HMG-box domains is a DNA clip to organize chloroplast nucleoids inProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Mitochondrial HMG-Box Containing Proteins: From Biochemical Properties to the Roles in Human Diseases.Biomolecules · 2020Review
- Evolutionary significance of the ring-like plastid nucleus in the primitive red alga Cyanidioschyzon merolae as revealed by drying.Protoplasma · 2020Article
- Novel clades of the HU/IHF superfamily point to unexpected roles in the eukaryotic centrosome, chromosome partitioning, and biologic conflicts.Cell cycle (Georgetown, Tex.) · 2017Article
- Review
- C-Terminal Region of Sulfite Reductase Is Important to Localize to Chloroplast Nucleoids in Land Plants.Genome biology and evolution · 2016Article
- Eukaryotic Components Remodeled Chloroplast Nucleoid Organization during the Green Plant Evolution.Genome biology and evolution · 2015Article
- Dark-induced senescence of barley leaves involves activation of plastid transglutaminases.Amino acids · 2015Article
- Dynamic composition, shaping and organization of plastid nucleoids.Frontiers in plant science · 2014Review
- Recent advances in the study of chloroplast gene expression and its evolution.Frontiers in plant science · 2014Review
- The HU protein is important for apicoplast genome maintenance and inheritance in Toxoplasma gondii.Eukaryotic cell · 2012Article
- Eukaryotic-type plastid nucleoid protein pTAC3 is essential for transcription by the bacterial-type plastid RNA polymerase.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Nucleoid-enriched proteomes in developing plastids and chloroplasts from maize leaves: a new conceptual framework for nucleoid functions.Plant physiology · 2012Article
- The YlmG protein has a conserved function related to the distribution of nucleoids in chloroplasts and cyanobacteria.BMC plant biology · 2010Article
- In vivo effects of NbSiR silencing on chloroplast development in Nicotiana benthamiana.Plant molecular biology · 2010Article
- Mechanisms of organelle division and inheritance and their implications regarding the origin of eukaryotic cells.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2010Review
- DNA organization by the apicoplast-targeted bacterial histone-like protein of Plasmodium falciparum.Nucleic acids research · 2008Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chloroplast DNA (cpDNA) is packed into discrete structures called chloroplast nucleoids (cp-nucleoids). The structure of cpDNA is thought to be important for its maintenance and regulation. In bacteria and mitochondria, histone-like proteins (such as HU and Abf2, respectively) are abundant and play important roles in DNA organization. However, a primary structural protein has yet to be found in cp-nucleoids. Here, we identified an abundant DNA binding protein from isolated cp-nucleoids of the primitive red alga Cyanidioschyzon merolae. The purified protein had sequence homology with the bacterial histone-like protein HU, and it complemented HU-lacking Escherichia coli mutants. The protein, called HC (histone-like protein of chloroplast), was encoded by a single gene (CmhupA) in the C. merolae chloroplast genome. Using immunofluorescence and immunoelectron microscopy, we demonstrated that HC was distributed uniformly throughout the entire cp-nucleoid. The protein was expressed constitutively throughout the cell and the chloroplast division cycle, and it was able to condense DNA. These results indicate that HC, a bacteria-derived histone-like protein, primarily organizes cpDNA into the nucleoid.
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