ArticleInfection and immunity2002
Molecular cloning and characterization of genes for Shigella sonnei form I O polysaccharide: proposed biosynthetic pathway and stable expression in a live salmonella vaccine vector.
Article in Infection and immunity, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 50 citations in OpenAlex.
- Development of a visual Adhesion/Invasion Inhibition Assay to assess the functionality ofFrontiers in immunology · 2024Article
- Maintenance of the Shigella sonnei Virulence Plasmid Is Dependent on Its Repertoire and Amino Acid Sequence of Toxin-Antitoxin Systems.Journal of bacteriology · 2022Article
- Development of multiplex cross displacement amplification combined with lateral flow biosensor assay for detection of virulentFrontiers in cellular and infection microbiology · 2022Article
- Article
- Additional Og-Typing PCR Techniques Targeting Escherichia coli-Novel andJournal of clinical microbiology · 2020Article
- Article
- Stable Chromosomal Expression of Shigella flexneri 2a and 3a O-Antigens in the Live Salmonella Oral Vaccine Vector Ty21a.Clinical and vaccine immunology : CVI · 2017Article
- A biologically conjugated polysaccharide vaccine delivered by attenuated Salmonella Typhimurium provides protection against challenge of avian pathogenic Escherichia coli O1 infection.Pathogens and disease · 2017Article
- Recombinant attenuated Salmonella Typhimurium with heterologous expression of the Salmonella Choleraesuis O-polysaccharide: high immunogenicity and protection.Scientific reports · 2017Article
- Two NovelFrontiers in cellular and infection microbiology · 2017Article
- Comparative Genomic and Phylogenetic Analysis of a Shiga Toxin ProducingFrontiers in cellular and infection microbiology · 2017Article
- Metabolic glycoengineering bacteria for therapeutic, recombinant protein, and metabolite production applications.Glycoconjugate journal · 2015Review
- An O antigen capsule modulates bacterial pathogenesis in Shigella sonnei.PLoS pathogens · 2015Article
- A novel multiplex PCR for the simultaneous detection of Salmonella enterica and Shigella species.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2014Article
- Haemophilus parainfluenzae expresses diverse lipopolysaccharide O-antigens using ABC transporter and Wzy polymerase-dependent mechanisms.International journal of medical microbiology : IJMM · 2013Article
- Genome Sequence of Salmonella enterica Serovar Typhi Oral Vaccine Strain Ty21a.Genome announcements · 2013Article
- The influence of riboflavin and nicotinic acid on Shigella sonnei colony conversion.Iranian journal of microbiology · 2011Article
- Structural characterization of ISCR8, ISCR22, and ISCR23, subgroups of IS91-like insertion elements.Antimicrobial agents and chemotherapy · 2010Article
- Anthrax protective antigen delivered by Salmonella enterica serovar Typhi Ty21a protects mice from a lethal anthrax spore challenge.Infection and immunity · 2009Article
- Predicted functions and linkage specificities of the products of the Streptococcus pneumoniae capsular biosynthetic loci.Journal of bacteriology · 2007Article
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Authors and funding
5 authors at 4 institutions in 1 country.
Funding
Abstract
The gene region for biosynthesis of Shigella sonnei form I O polysaccharide (O-Ps) and flanking sequences, totaling >18 kb, was characterized by deletion analysis to define a minimal construct for development of Salmonella-based live vaccine vector strains. Lipopolysaccharide (LPS) expression and DNA sequence studies of plasmid deletion derivatives indicated form I O-Ps expression from a 12.3-kb region containing a putative promoter and 10 contiguous open reading frames (ORFs), one of which is the transposase of IS630. A detailed biosynthetic pathway, consistent with the predicted functions of eight of the nine essential ORFs and the form I O-Ps structure, is proposed. Further sequencing identified partial IS elements (i.e., IS91 and IS630) and wzz upstream of the form I coding region and a fragment of aqpZ and additional full or partial IS elements (i.e., IS629, IS91, and IS911) downstream of this region. The stability of plasmid-based form I O-Ps expression was greater from low-copy vectors than from high-copy vectors and was enhanced by deletion of the downstream IS91 from plasmid inserts. Both core-linked (i.e., LPS) and non-core-linked (i.e., capsule-like) surface expression of form I O-Ps were detected by Western blotting and silver staining of polyacrylamide gel electrophoresis-separated Shigella and Escherichia coli extracts. However, salmonellae, which have a core that is chemically dissimilar to that of shigellae, expressed only non-core-linked surface-associated form I O-Ps. Finally, attenuated Salmonella enterica serovar Typhi live vaccine vector candidates, containing minimal-sized form I operon constructs, elicited immune protection in mice against virulent S. sonnei challenge, thereby supporting the promise of live, oral vaccines for the prevention of shigellosis.
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