ArticleInfection and immunity2002
Loss of regulatory protein RfaH attenuates virulence of uropathogenic Escherichia coli.
Article in Infection and immunity, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed, 95 citations in OpenAlex.
- Unmasking pathogen traits for chronic colonization in neurogenic bladder.Cell reports · 2026Article
- Article
- Evaluation of the in vitro effects of concentrations of antibiotics on three Enterobacteriaceae isolates.World journal of microbiology & biotechnology · 2024Article
- RfaH contributes to maximal colonization and full virulence of hypervirulentFrontiers in cellular and infection microbiology · 2024Article
- Phage resistance formation and fitness costs of hypervirulentFrontiers in microbiology · 2023Article
- Article
- Putative transcription antiterminator RfaH contributes to Erwinia amylovora virulence.Molecular plant pathology · 2022Article
- Phage Resistance Accompanies Reduced Fitness of Uropathogenic Escherichia coli in the Urinary Environment.mSphere · 2022Article
- RfaH May Oppose Silencing by H-NS and YmoA Proteins during Transcription Elongation.Journal of bacteriology · 2022Article
- "Omics" Technologies - What Have They Told Us About UropathogenicFrontiers in cellular and infection microbiology · 2022Review
- Control of a programmed cell death pathway in Pseudomonas aeruginosa by an antiterminator.Nature communications · 2021Article
- Rcs Phosphorelay Responses to Truncated Lipopolysaccharide-Induced Cell Envelope Stress inMolecules (Basel, Switzerland) · 2020Article
- NusG, an Ancient Yet Rapidly Evolving Transcription Factor.Frontiers in microbiology · 2020Review
- Ancient Transcription Factors in the News.mBio · 2019Review
- Regulation of hemolysin in uropathogenic Escherichia coli fine-tunes killing of human macrophages.Virulence · 2018Article
- In silico discovery of small molecules that inhibit RfaH recruitment to RNA polymerase.Molecular microbiology · 2018Article
- A new role for Zinc limitation in bacterial pathogenicity: modulation of α-hemolysin from uropathogenic Escherichia coli.Scientific reports · 2018Article
- Molecular Epidemiology of Extraintestinal PathogenicEcoSal Plus · 2018Review
- Differential impact of lipopolysaccharide defects caused by loss of RfaH in Yersinia pseudotuberculosis and Yersinia pestis.Scientific reports · 2017Article
- Flipping states: a few key residues decide the winning conformation of the only universally conserved transcription factor.Nucleic acids research · 2017Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RfaH is a regulatory protein in Escherichia coli and Salmonella enterica serovar Typhimurium. Although it enhances expression of different factors that are proposed to play a role in bacterial virulence, a direct effect of RfaH on virulence has not been investigated so far. We report that inactivation of rfaH dramatically decreases the virulence of uropathogenic E. coli strain 536 in an ascending mouse model of urinary tract infection. The mortality rate caused by the wild-type strain in this assay is 100%, whereas that of its isogenic rfaH mutant does not exceed 18%. In the case of coinfection, the wild-type strain 536 shows higher potential to colonize the urinary tract even when it is outnumbered 100-fold by its rfaH mutant in the inoculum. In contrast to the wild-type strain, serum resistance of strain 536rfaH::cat is fully abolished. Furthermore, we give evidence that, besides a major decrease in the amount of hemin receptor ChuA (G. Nagy, U. Dobrindt, M. Kupfer, L. Emody, H. Karch, and J. Hacker, Infect. Immun. 69:1924-1928, 2001), loss of the RfaH protein results in an altered lipopolysaccharide phenotype as well as decreased expression of K15 capsule and alpha-hemolysin, whereas levels of other pathogenicity factors such as siderophores, flagella, Prf, and S fimbriae appear to be unaltered in strain 536rfaH::cat in comparison to the wild-type strain. trans complementation of the mutant strain with the rfaH gene restores wild-type levels of the affected virulence factors and consequently restitutes virulence in the mouse model of ascending urinary tract infection.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.