ArticleThe Biochemical journal2002
Urokinase upregulates matrix metalloproteinase-9 expression in THP-1 monocytes via gene transcription and protein synthesis.
Article in The Biochemical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 54 citations in OpenAlex.
- Identification and characterisation of subtype-specific anti-N-CoR OSGEP protease in acute myeloid leukaemia (AML-M5) cell lineage.Biotechnology letters · 2025Article
- Matrix-metalloproteinases as targets for controlled delivery in cancer: An analysis of upregulation and expression.Journal of controlled release : official journal of the Controlled Release Society · 2017Review
- Urokinase-type plasminogen activator resulting from endometrial carcinogenesis enhances tumor invasion and correlates with poor outcome of endometrial carcinoma patients.Scientific reports · 2015Article
- Decreased expression of the long non-coding RNA FENDRR is associated with poor prognosis in gastric cancer and FENDRR regulates gastric cancer cell metastasis by affecting fibronectin1 expression.Journal of hematology & oncology · 2014Article
- Urokinase plasminogen activator gene deficiency inhibits fracture cartilage remodeling.Journal of bone and mineral metabolism · 2014Article
- Beneficial Effects of Ocimum gratissimum Aqueous Extract on Rats with CCl(4)-Induced Acute Liver Injury.Evidence-based complementary and alternative medicine : eCAM · 2012Article
- Down-regulation of survivin suppresses uro-plasminogen activator through transcription factor JunB.Experimental & molecular medicine · 2011Article
- Collateral vessel growth induced by femoral artery ligature is impaired by denervation.Molecular and cellular biochemistry · 2011Article
- Matrix metalloproteinase-9 and -2 expression in the olfactory bulb following methyl bromide gas exposure.Chemical senses · 2010Article
- Formation of cardiovascular tubes in invertebrates and vertebrates.Cellular and molecular life sciences : CMLS · 2010Review
- Inhibition of histone deacetylase activity down-regulates urokinase plasminogen activator and matrix metalloproteinase-9 expression in gastric cancer.Molecular and cellular biochemistry · 2010Article
- Matrix metalloproteinase-9 regulates tumor cell invasion through cleavage of protease nexin-1.Cancer research · 2010Article
- Association of Ang-2 with integrin beta 2 controls Ang-2/PDGF-BB-dependent upregulation of human peripheral blood monocyte fibrinolysis.Inflammation · 2009Article
- Induction of the plasminogen activator system by mechanical stimulation of human bronchial epithelial cells.American journal of respiratory cell and molecular biology · 2006Article
- Text mining of full-text journal articles combined with gene expression analysis reveals a relationship between sphingosine-1-phosphate and invasiveness of a glioblastoma cell line.BMC bioinformatics · 2006Article
- Phenotypic conversion of human mammary carcinoma cells by autocrine human growth hormone.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Arteriogenesis versus angiogenesis: similarities and differences.Journal of cellular and molecular medicineReview
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Urokinase-type plasminogen activator (uPA) is suggested to exert its proliferatory, migratory and invasive action through binding with its membrane receptor, promoting pericellular proteolysis and mediating cell signal transduction. One of the possible actions of urokinase can be related to the regulation of activity and/or the expression of proteolytic enzymes participating in extracellular matrix degradation. In the present study, the role of uPA in regulating matrix metalloproteinase (MMP) expression and release by the monocyte cell line THP-1 was investigated. Recombinant uPA induced the release of MMP9/gelatinase B, as detected by zymography and Western blotting, and this release was abolished by actinomycin D and cycloheximide (inhibitors of DNA transcription and protein synthesis) and partially suppressed by monensin (an inhibitor of secretion). Proteolytically inactive urokinase with substitution of His(204) for Gln was able to reproduce about 70% of the effect induced by the wild-type recombinant uPA. The reverse transcription-PCR and Northern blot data indicated that the action of r-uPA on THP-1 cells resulted in formation of MMP9 mRNA, which depended on time, within 6-48 h, of the cell incubation with r-uPA. These results suggest that urokinase upregulates MMP9 expression in monocytes via MMP9 gene transcription and protein biosynthesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.