ArticlePlant physiology2002
Regulation of squalene synthase, a key enzyme of sterol biosynthesis, in tobacco.
Article in Plant physiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 103 citations in OpenAlex.
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- Cyclodextrins Increase Triterpene Production inPlants (Basel, Switzerland) · 2022Article
- MaizeFrontiers in plant science · 2021Article
- The Sterol Trafficking Pathway inFrontiers in plant science · 2021Article
- Genome-Wide HMG Family Investigation and Its Role in Glycoalkaloid Accumulation in Wild Tuber-BearingLife (Basel, Switzerland) · 2020Article
- Transcriptome analysis of Clinopodium gracile (Benth.) Matsum and identification of genes related to Triterpenoid Saponin biosynthesis.BMC genomics · 2020Article
- Differential Impact of Salinity Stress on Seeds Minerals, Storage Proteins, Fatty Acids, and Squalene Composition of New Quinoa Genotype, Grown in Hyper-Arid Desert Environments.Frontiers in plant science · 2020Article
- Genome-Wide Association Study for Squalene Contents and Functional Haplotype Analysis in Rice.ACS omega · 2019Article
- Tomato locule number and fruit size controlled by natural alleles ofPlant direct · 2019Article
- Positive Selection of Squalene Synthase in Cucurbitaceae Plants.International journal of genomics · 2019Article
- Squalene synthase cloning and functional identification in wintersweet plant (Chimonanthus zhejiangensis).Botanical studies · 2018Article
- Effect of terbinafine on the biosynthetic pathway of isoprenoid compounds in carrot suspension cultured cells.Plant cell reports · 2018Article
- Functional characterization of squalene synthase and squalene epoxidase inPlant direct · 2018Article
- Improved fruit α-tocopherol, carotenoid, squalene and phytosterol contents through manipulation of Brassica juncea 3-HYDROXY-3-METHYLGLUTARYL-COA SYNTHASE1 in transgenic tomato.Plant biotechnology journal · 2018Article
- Transcriptome analysis reveals metabolic alteration due to consecutive monoculture and abiotic stress stimuli in Rehamannia glutinosa Libosch.Plant cell reports · 2017Article
- Phytogeographic and genetic variation inPeerJ · 2016Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Squalene synthase (SS) represents a putative branch point in the isoprenoid biosynthetic pathway capable of diverting carbon flow specifically to the biosynthesis of sterols and, hence, is considered a potential regulatory point for sterol metabolism. For example, when plant cells grown in suspension culture are challenged with fungal elicitors, suppression of sterol biosynthesis has been correlated with a reduction in SS enzyme activity. The current study sought to correlate changes in SS enzyme activity with changes in the level of the corresponding protein and mRNA. Using an SS-specific antibody, the initial suppression of SS enzyme activity in elicitor-challenged cells was not reflected by changes in the absolute level of the corresponding polypeptide, implicating a post-translational control mechanism for this enzyme activity. In comparison, the absolute level of the SS mRNA did decrease approximately 5-fold in the elicitor-treated cells, which is suggestive of decreased transcription of the SS gene. Study of SS in intact plants was also initiated by measuring the level of SS enzyme activity, the level of the corresponding protein, and the expression of SS gene promoter-reporter gene constructs in transgenic plants. SS enzyme activity, polypeptide level, and gene expression were all localized predominately to the shoot apical meristem, with much lower levels observed in leaves and roots. These later results suggest that sterol biosynthesis is localized to the apical meristems and that apical meristems may be a source of sterols for other plant tissues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.