ArticlePlant physiology2002
Immunohistochemistry of active gibberellins and gibberellin-inducible alpha-amylase in developing seeds of morning glory.
Article in Plant physiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Dual-function DEFENSIN 8 mediates phloem cadmium unloading and accumulation in rice grains.Plant physiology · 2023Article
- Transcriptome and Coexpression Network Analyses Provide In-Sights into the Molecular Mechanisms of Hydrogen Cyanide Synthesis during Seed Development in Common Vetch (International journal of molecular sciences · 2022Article
- Transcriptome Analysis Reveals Key Pathways and Candidate Genes Controlling Seed Development and Size in Ricebean (Frontiers in genetics · 2021Article
- Global transcriptome analysis and identification of genes involved in nutrients accumulation during seed development of rice tartary buckwheat (Fagopyrum Tararicum).Scientific reports · 2017Article
- Proper gibberellin localization in vascular tissue is required to regulate adventitious root development in tobacco.Journal of experimental botany · 2013Article
- Tissue-specific regulation of gibberellin biosynthesis in developing pea seeds.Plant physiology · 2011Article
- A genome survey of Moniliophthora perniciosa gives new insights into Witches' Broom Disease of cacao.BMC genomics · 2008Article
- New insights into the complex and coordinated transcriptional regulation networks underlying rice seed development through cDNA chip-based analysis.Plant molecular biology · 2005Article
- Expression studies of gibberellin oxidases in developing pumpkin seeds.Plant physiology · 2003Article
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Authors and funding
5 authors.
Funding
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Abstract
Gibberellins (GAs) in developing seeds of morning glory (Pharbitis nil) were quantified and localized by immunostaining. The starch grains began to be digested after the GA contents had increased and reached a plateau. Immunohistochemical staining with the antigibberellin A(1)-methyl ester-antiserum, which has high affinity to biologically active GAs, showed that GA(1) and/or GA(3) were localized around starch grains in the integument of developing young seeds, suggesting the participation of GA-inducible alpha-amylase in this digestion. We isolated an alpha-amylase cDNA (PnAmy1) that was expressed in the immature seeds, and using an antibody raised against recombinant protein, it was shown that PnAmy1 was expressed in the immature seeds. GA responsiveness of PnAmy1 was shown by treating the young fruits 9 d after anthesis with GA(3). RNA-blot and immunoblot analyses showed that PnAmy1 emerged soon after the rapid increase of GA(1/3). An immunohistochemical analysis of PnAmy1 showed that it, like the seed GA(1/3), was also localized around starch grains in the integument of developing young seeds. The localization of GA(1/3) in the integument coincident with the expression of PnAmy1 suggests that both function as part of a process to release sugars for translocation or for the further development of the seeds.
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