ArticleThe EMBO journal2002
Projection structure at 8 A resolution of the melibiose permease, an Na-sugar co-transporter from Escherichia coli.
Article in The EMBO journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 47 citations in OpenAlex.
- Ins and Outs of Rocker Switch Mechanism in Major Facilitator Superfamily of Transporters.Membranes · 2023Review
- Structures and General Transport Mechanisms by the Major Facilitator Superfamily (MFS).Chemical reviews · 2021Article
- Two-Dimensional Crystallization Procedure, from Protein Expression to Sample Preparation.BioMed research international · 2015Review
- Reduced Na+ affinity increases turnover of Salmonella enterica serovar Typhimurium MelB.Journal of bacteriology · 2012Article
- Role of Gly117 in the cation/melibiose symport of MelB of Salmonella typhimurium.Biochemistry · 2012Article
- Mechanism of melibiose/cation symport of the melibiose permease of Salmonella typhimurium.The Journal of biological chemistry · 2011Article
- Structural insights into the activation mechanism of melibiose permease by sodium binding.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- A 3D structure model of the melibiose permease of Escherichia coli represents a distinctive fold for Na+ symporters.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Alteration of sugar-induced conformational changes of the melibiose permease by mutating Arg141 in loop 4-5.Biophysical journal · 2009Article
- FTIR spectroscopy of secondary-structure reorientation of melibiose permease modulated by substrate binding.Biophysical journal · 2008Article
- Changes in secondary structures and acidic side chains of melibiose permease upon cosubstrates binding.Biophysical journal · 2006Article
- Elucidation of substrate binding interactions in a membrane transport protein by mass spectrometry.The EMBO journal · 2003Article
- An approach to membrane protein structure without crystals.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ion-coupled sugar membrane symporter or co-transporter melibiose permease (MelB), responsible for alpha-galactoside accumulation in Escherichia coli, is a representative member of the glycoside-pentoside- hexuronide family of the vast class of electrochemical potential-driven porters. Pure solubilized preparations of a MelB recombinant protein were subjected to two-dimensional crystallization trials and several crystal forms were observed. Two of these appeared as large wide tubes suitable for analysis by electron crystallography. Flattened tubes on carbon support film, embedded in amorphous ice prior to electron cryomicroscopy, showed two-sided plane group symmetries P12(1) or P222(1), with unit cell dimensions a = 89.9 A, b = 51.6 A, gamma = 91.9 degrees and a = 188.9 A, b = 48.8 A, gamma = 90 degrees, respectively. The projection map from the P222(1 )crystals at 8 A resolution displayed an asymmetric protein unit consisting of two domains lining a central and curve-shaped cleft. Together, the MelB monomer could host the 12 predicted transmembrane alpha-helices. Overall, the MelB helix packing arrangement compared more favorably with that of the Na(+)/H(+) antiporter NhaA than that of the oxalate antiporter.
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