ArticleThe American journal of pathology2002
Microinjection of cathepsin d induces caspase-dependent apoptosis in fibroblasts.
Article in The American journal of pathology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
58 citing papers in PubMed, 190 citations in OpenAlex.
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- Death Pathways of Cancer Cells Modulated by Surface Molecule Density on Gold Nanorods.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- Late endosomes promote microglia migration via cytosolic translocation of immature protease cathD.Science advances · 2020Article
- Lysosomal Hydrolase Cathepsin D Non-proteolytically Modulates Dendritic Morphology in Drosophila.Neuroscience bulletin · 2020Article
- Lowering Endogenous Cathepsin D Abundance Results in Reactive Oxygen Species Accumulation and Cell Senescence.Molecular & cellular proteomics : MCP · 2017Article
- Cancer with low cathepsin D levels is susceptible to vacuolar (HCancer science · 2017Article
- Empirical Modeling of Physiochemical Immune Response of Multilayer Zinc Oxide Nanomaterials under UV Exposure to Melanoma and Foreskin Fibroblasts.Scientific reports · 2017Article
- The Anti-Apoptotic Properties of APEX1 in the Endothelium Require the First 20 Amino Acids and Converge on Thioredoxin-1.Antioxidants & redox signaling · 2017Article
- Aberrant DR5 transport through disruption of lysosomal function suggests a novel mechanism for receptor activation.Oncotarget · 2016Article
- Lysosomal protease cathepsin D; a new driver of apoptosis during acute kidney injury.Scientific reports · 2016Article
- Lysosomal membrane permeabilization is involved in oxidative stress-induced apoptotic cell death in LAMP2-deficient iPSCs-derived cerebral cortical neurons.Biochemistry and biophysics reports · 2016Article
- PIG7 promotes leukemia cell chemosensitivity via lysosomal membrane permeabilization.Oncotarget · 2016Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recent reports have indicated that enzymes such as cathepsins D and B are translocated from lysosomal compartments to the cytosol early during apoptosis. We have previously noted that a translocation of cathepsins D and B occur before cytochrome c release and caspase activation in cardiomyocytes and human fibroblasts during oxidative stress-induced apoptosis. In the present report, we use a microinjection technique to investigate if cytosolic location of the cathepsins D and B are important for induction of apoptosis. We found that microinjection of cathepsin D into the cytosol of human fibroblasts caused apoptosis, which was detected as changes in distribution of cytochrome c, cell shrinkage, activation of caspases, chromatin condensation, and formation of pycnotic nuclei. No apoptosis was, however, induced by microinjection of cathepsin B. Moreover, apoptosis was prevented in fibroblasts pretreated with a caspase-3-like inhibitor, and also when microinjected with cathepsin D mixed with the cathepsin D inhibitor, pepstatin A. These results show that cytosolic cathepsin D can act as a proapoptotic mediator upstream of cytochrome c release and caspase activation in human fibroblasts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.