ArticleBMC microbiology2002
Determination of PCR efficiency in chelex-100 purified clinical samples and comparison of real-time quantitative PCR and conventional PCR for detection of Chlamydia pneumoniae.
Article in BMC microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 70 citations in OpenAlex.
- Real-Time PCR Protocol for Detection and Quantification of Three Pathogenic Members of the Vibrionaceae Family.Microorganisms · 2022Article
- Using Species a Rotavirus Reverse Genetics to Engineer Chimeric Viruses Expressing SARS-CoV-2 Spike Epitopes.Journal of virology · 2022Article
- Method for improving the quality of genomic DNA obtained from minute quantities of tissue and blood samples using Chelex 100 resin.Biological procedures online · 2018Article
- Article
- A new method for quantitative real-time polymerase chain reaction data analysis.Journal of computational biology : a journal of computational molecular cell biology · 2013Article
- An improvement of the 2ˆ(-delta delta CT) method for quantitative real-time polymerase chain reaction data analysis.Biostatistics, bioinformatics and biomathematics · 2013Article
- Downregulation of caspase-10 predicting poor survival after resection of stage II colorectal cancer.International journal of colorectal disease · 2011Article
- Evaluation of a quantitative real-time PCR assay to measure HIV-specific mucosal CD8+ T cell responses in the cervix.PloS one · 2010Article
- Isolation of Chlamydia pneumoniae from serum samples of the patients with acute coronary syndrome.International journal of medical sciences · 2010Article
- Use of pJANUS-02-001 as a calibrator plasmid for Roundup Ready soybean event GTS-40-3-2 detection: an interlaboratory trial assessment.Analytical and bioanalytical chemistry · 2010Article
- The mRNA and protein expression of A-kinase anchor proteins 13 in human colorectal cancer.Clinical and experimental medicine · 2010Article
- Real-time PCR machine system modeling and a systematic approach for the robust design of a real-time PCR-on-a-chip system.Sensors (Basel, Switzerland) · 2010Article
- Real-time PCR in clinical microbiology: applications for routine laboratory testing.Clinical microbiology reviews · 2006Review
- Standardisation of data from real-time quantitative PCR methods - evaluation of outliers and comparison of calibration curves.BMC biotechnology · 2005Article
- Screening for sexually transmitted infection pathogens in semen samples.The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2005Article
- Diagnosis and assessment of trachoma.Clinical microbiology reviews · 2004Review
- A high-throughput method to monitor the expression of microRNA precursors.Nucleic acids research · 2004Article
- Article
- QUANTITATIVE VS. CONVENTIONAL PCR FOR DETECTION OF HUMAN ADENOVIRUSES IN WATER AND SEDIMENT SAMPLES.Revista do Instituto de Medicina Tropical de Sao PauloArticle
- Comparison of PCR-based methods for the simultaneous detection of Neisseria meningitidis, Haemophilus influenzae, and Streptococcus pneumoniae in clinical samples.The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious DiseasesArticle
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundChlamydia pneumoniae infection has been detected by serological methods, but PCR is gaining more interest. A number of different PCR assays have been developed and some are used in combination with serology for diagnosis. Real-time PCR could be an attractive new PCR method; therefore it must be evaluated and compared to conventional PCR methods.
resultsWe compared the performance of a newly developed real-time PCR with a conventional PCR method for detection of C. pneumoniae. The PCR methods were tested on reference samples containing C. pneumoniae DNA and on 136 nasopharyngeal samples from patients with chronic cough. We found the same detection limit for the two methods and clinical performance was equal for the real-time PCR and for the conventional PCR method, although only three samples tested positive. To investigate whether the low prevalence of C. pneumoniae among patients with chronic cough was caused by suboptimal PCR efficiency in the samples, PCR efficiency was determined based on the real-time PCR. Seventeen of twenty randomly selected clinical samples had similar PCR efficiency to samples containing pure genomic C. pneumoniae DNA.
conclusionThese results indicate that the performance of real-time PCR is comparable to that of conventional PCR, but this needs to be confirmed further. Real-time PCR can be used to investigate the PCR efficiency which gives a rough estimate of how well the real-time PCR assay work in a specific sample type. Suboptimal PCR efficiency of PCR is not a likely explanation for the low positivity rate of C. pneumoniae in patients with chronic cough.
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