ArticleThe Journal of cell biology2002
Dual-wavelength fluorescent speckle microscopy reveals coupling of microtubule and actin movements in migrating cells.
Article in The Journal of cell biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 96 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
96 citing papers in PubMed, 209 citations in OpenAlex.
- Microtubule-driven cell shape changes and actomyosin flow synergize to position the centrosome.The Journal of cell biology · 2025Article
- Cryo-ET of actin cytoskeleton and membrane structure in lamellipodia formation using optogenetics.iScience · 2025Article
- A simple active fluid model unites cytokinesis, cell crawling, and axonal outgrowth.bioRxiv : the preprint server for biology · 2024Article
- A simple active fluid model unites cytokinesis, cell crawling, and axonal outgrowth.Frontiers in cell and developmental biology · 2024Article
- Morphological and cytoskeleton changes in cells after EMT.Scientific reports · 2023Article
- CLASP2 facilitates dynamic actin filament organization along the microtubule lattice.Molecular biology of the cell · 2023Article
- PRR11 induces filopodia formation and promotes cell motility via recruiting ARP2/3 complex in non-small cell lung cancer cells.Genes & diseases · 2022Article
- Triggering Cation-Induced Contraction of Cytoskeleton Networks via Microfluidics.Frontiers in physics · 2020Article
- Review
- Profilin choreographs actin and microtubules in cells and cancer.International review of cell and molecular biology · 2020Article
- Microtubules in cell migration.Essays in biochemistry · 2019Review
- Varying crosslinking motifs drive the mesoscale mechanics of actin-microtubule composites.Scientific reports · 2019Article
- Interaction of microtubules and actin during the post-fusion phase of exocytosis.Scientific reports · 2019Article
- Microtubules promote intercellular contractile force transmission during tissue folding.The Journal of cell biology · 2019Article
- Actin-microtubule crosstalk in cell biology.Nature reviews. Molecular cell biology · 2019Review
- Unbiased pattern analysis reveals highly diverse responses of cytoskeletal systems to cyclic straining.PloS one · 2019Article
- BIG2-ARF1-RhoA-mDia1 Signaling Regulates Dendritic Golgi Polarization in Hippocampal Neurons.Molecular neurobiology · 2018Article
- Mammalian Plakins, Giant Cytolinkers: Versatile Biological Functions and Roles in Cancer.International journal of molecular sciences · 2018Review
- A cytoskeletal clutch mediates cellular force transmission in a soft, three-dimensional extracellular matrix.Molecular biology of the cell · 2017Article
- Dynamic microtubules regulate cellular contractility during T-cell activation.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
36 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Interactions between microtubules (MTs) and filamentous actin (f-actin) are involved in directed cell locomotion, but are poorly understood. To test the hypothesis that MTs and f-actin associate with one another and affect each other's organization and dynamics, we performed time-lapse dual-wavelength spinning-disk confocal fluorescent speckle microscopy (FSM) of MTs and f-actin in migrating newt lung epithelial cells. F-actin exhibited four zones of dynamic behavior: rapid retrograde flow in the lamellipodium, slow retrograde flow in the lamellum, anterograde flow in the cell body, and no movement in the convergence zone between the lamellum and cell body. Speckle analysis showed that MTs moved at the same trajectory and velocity as f-actin in the cell body and lamellum, but not in the lamellipodium or convergence zone. MTs grew along f-actin bundles, and quiescent MT ends moved in association with f-actin bundles. These results show that the movement and organization of f-actin has a profound effect on the dynamic organization of MTs in migrating cells, and suggest that MTs and f-actin bind to one another in vivo.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.