ArticleThe Biochemical journal2002
Binding of C-reactive protein to modified low-density-lipoprotein particles: identification of cholesterol as a novel ligand for C-reactive protein.
Article in The Biochemical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 66 citations in OpenAlex.
- C-Reactive Protein in Atherosclerosis-More than a Biomarker, but not Just a Culprit.Reviews in cardiovascular medicine · 2023Review
- C-Reactive Protein: Pathophysiology, Diagnosis, False Test Results and a Novel Diagnostic Algorithm for Clinicians.Diseases (Basel, Switzerland) · 2023Review
- An overview onHeliyon · 2020Review
- Functionality of C-Reactive Protein for Atheroprotection.Frontiers in immunology · 2019Review
- Recognition functions of pentameric C-reactive protein in cardiovascular disease.Mediators of inflammation · 2014Review
- Exposing a hidden functional site of C-reactive protein by site-directed mutagenesis.The Journal of biological chemistry · 2012Article
- Atherosclerosis-related functions of C-reactive protein.Cardiovascular & hematological disorders drug targets · 2010Article
- Study of the interaction of the C-reactive protein monomer with the U937 monocyte.Cellular & molecular biology letters · 2010Article
- Binding of the monomeric form of C-reactive protein to enzymatically-modified low-density lipoprotein: effects of phosphoethanolamine.Clinica chimica acta; international journal of clinical chemistry · 2009Article
- CRP and the risk of atherosclerotic events.Seminars in immunopathology · 2009Review
- Pattern recognition by pentraxins.Advances in experimental medicine and biology · 2009Review
- Phosphoethanolamine-complexed C-reactive protein: a pharmacological-like macromolecule that binds to native low-density lipoprotein in human serum.Clinica chimica acta; international journal of clinical chemistry · 2008Article
- C-reactive protein-bound enzymatically modified low-density lipoprotein does not transform macrophages into foam cells.Journal of immunology (Baltimore, Md. : 1950) · 2008Article
- The connection between C-reactive protein and atherosclerosis.Annals of medicine · 2008Review
- Physical activity and high-sensitivity C-reactive protein.Sports medicine (Auckland, N.Z.) · 2006Review
- Plasma kinetics of free and esterified cholesterol in familial hypercholesterolemia: effects of simvastatin.Lipids · 2005Article
- CRP after 2004.Molecular immunology · 2005Review
- Evidence for C-reactive protein's role in (CRP) vascular disease: atherothrombosis, immuno-regulation and CRP.Journal of thrombosis and thrombolysis · 2004Review
- Induction of glycosylation in human C-reactive protein under different pathological conditions.The Biochemical journal · 2003Article
- Oxidized phosphatidylcholines: pattern recognition ligands for multiple pathways of the innate immune response.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
C-reactive protein (CRP), an acute-phase reactant, is present in atherosclerotic human arterial intima in association with lipids. In the present work we studied interactions between CRP and LDL on microtitre wells, where either CRP or LDL was immobilized. LDL was modified by vortex-mixing, oxidation, or by lipolysis with phospholipase A(2) or with sphingomyelinase or a combination of trypsin and cholesterol esterase. We found that CRP bound only to LDL modified by trypsin/cholesterol esterase or by sphingomyelinase and that this binding was Ca(2+)-dependent. In these two forms of modified LDL, non-esterified cholesterol was susceptible to cholesterol oxidase, indicating exposure of non-esterified cholesterol on particle surfaces and suggesting a role for non-esterified cholesterol in mediating CRP binding. Consistent with this hypothesis were the following findings: (i) increasing the amount of non-esterified cholesterol in LDL with cyclodextrin increased, and decreasing its amount decreased, the binding of CRP to LDL; (ii) modification of non-esterified cholesterol in LDL by cholesterol oxidase decreased the binding of CRP to LDL; and (iii) CRP bound to purified non-esterified cholesterol. The binding was Ca(2+)-dependent and could be competed out with phosphocholine. Taken together, these findings suggest that CRP can bind to modified lipoproteins, notably to the non-esterified cholesterol on their surface. These interactions may be related to the suggested role of CRP in the local inflammation present in atherosclerotic plaques.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.