ArticleMolecular and cellular biology2002
Granuphilin modulates the exocytosis of secretory granules through interaction with syntaxin 1a.
Article in Molecular and cellular biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 97 citations in OpenAlex.
- A Syd and RUFY dynein adaptor complex mediates axonal circulation of dense core vesicles.The Journal of cell biology · 2026Article
- The a subunit isoforms of V-ATPase are involved in glucose-dependent trafficking of insulin granules.Scientific reports · 2025Article
- Multiple pathways and independent functional pools in insulin granule exocytosis.Genes to cells : devoted to molecular & cellular mechanisms · 2023Review
- Functional hierarchy among different Rab27 effectors involved in secretory granule exocytosis.eLife · 2023Article
- Membrane Fusion and SNAREs: Interaction with Ras Proteins.International journal of molecular sciences · 2022Review
- Munc13b stimulus-dependently accumulates on granuphilin-mediated, docked granules prior to fusion.Cell structure and function · 2022Article
- In vivo Roles of Rab27 and Its Effectors in Exocytosis.Cell structure and function · 2021Review
- Targeting Small GTPases and Their Prenylation in Diabetes Mellitus.Journal of medicinal chemistry · 2021Review
- Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men.Physiological reviews · 2018Review
- Proteomic analysis of insulin secretory granules in INS-1 cells by protein correlation profiling.Biophysics reports · 2018Article
- Granuphilin exclusively mediates functional granule docking to the plasma membrane.Scientific reports · 2016Article
- Increase of Calcium Sensing Receptor Expression Is Related to Compensatory Insulin Secretion during Aging in Mice.PloS one · 2016Article
- Interplay between Rab27a effectors in pancreatic β-cells.World journal of diabetes · 2015Review
- Article
- Activated Cdc42-bound IQGAP1 determines the cellular endocytic site.Molecular and cellular biology · 2013Article
- Regulation of vesicular trafficking and leukocyte function by Rab27 GTPases and their effectors.Journal of leukocyte biology · 2013Review
- The secretion of von Willebrand factor from endothelial cells; an increasingly complicated story.Journal of thrombosis and haemostasis : JTH · 2013Review
- The Rab27a effector exophilin7 promotes fusion of secretory granules that have not been docked to the plasma membrane.Molecular biology of the cell · 2013Article
- Article
- Epigenetics: the missing link to understanding β-cell dysfunction in the pathogenesis of type 2 diabetes.Epigenetics · 2012Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The molecular mechanism for the regulated exocytosis of dense-core granules in endocrine cells remains relatively uncharacterized compared to that of synaptic vesicles in neurons. A novel set of Rab and its effector, Rab27a/granuphilin, which is localized on insulin granules in pancreatic beta cells, was recently identified. Here we demonstrate that granuphilin directly binds to syntaxin 1a on the plasma membrane, and this interaction is regulated by Rab27a. Granuphilin shows affinity to syntaxin 1a with a closed conformation but not to mutant syntaxin 1a, which adopts an open conformation constitutively. Overexpression of granuphilin significantly enhances basal insulin secretion but profoundly inhibits high K(+)-induced insulin secretion. The effect of granuphilin on insulin secretion was impaired by its mutation that disrupts the binding to either Rab27a or syntaxin 1a. Thus, granuphilin is the first regulator in the exocytotic pathway that functions by directly connecting two critical vesicle transport proteins, Rab and SNARE.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.