ArticleBMC infectious diseases2002
A quantitative PCR (TaqMan) assay for pathogenic Leptospira spp.
Article in BMC infectious diseases, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 132 papers, 2 of them syntheses that pooled it.
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Who cites it
132 citing papers in PubMed, 2 syntheses or guidelines pooled it, 311 citations in OpenAlex.
- Diagnostic accuracy of genetic markers and nucleic acid techniques for the detection of Leptospira in clinical samples: A meta-analysis.PLoS neglected tropical diseases · 2020Pooled it
- Meta-analysis to estimate the load of Leptospira excreted in urine: beyond rats as important sources of transmission in low-income rural communities.BMC research notes · 2017Pooled it
- Application of an integrated outbreak management plan for the control of leptospirosis in dairy cattle herds.Epidemiology and infection · 2014Trial
- A systematic review ofOne health (Amsterdam, Netherlands) · 2026Article
- Longitudinal Survey of Pathogenic Leptospira Species at a Major Park in Sydney, Australia.EcoHealth · 2026Article
- Deciphering the "Doxycycline Deficiency Syndrome": Defining the Characteristic Clinical, Laboratory and Imaging Findings in Patients Presenting with Leptospirosis, Q Fever and Rickettsial Diseases in Far North Queensland, Tropical Australia.Tropical medicine and infectious disease · 2026Article
- Serological and molecular detection of Leptospira spp. in ring-tailed coatis (Nasua nasua) from an Ecological Park.Veterinary research communications · 2026Article
- Diagnosis of leptospirosis in animals: challenges and perspectives.Journal of clinical microbiology · 2026Review
- Article
- Urine PCR testing as an effective method for early diagnosis of leptospirosis.Microbiology spectrum · 2025Article
- Evolution of human leptospirosis in French Guiana, 2016-2022.PLoS neglected tropical diseases · 2025Article
- Chronic Leptospirosis in a Breeding Bull: A Case Report.Microorganisms · 2025Article
- Role of individual and population heterogeneity in shaping dynamics of multi-pathogen shedding in an island endemic bat.PLoS pathogens · 2025Article
- Performance of electrochemical aptasensor as antigen test in clinical samples for early diagnosis of leptospirosis.Scientific reports · 2025Article
- Diagnostic Advances in Leptospirosis: A Comparative Analysis of Paraclinical Tests with a Focus on PCR.Microorganisms · 2025Review
- Production of recombinant electron transfer flavoprotein beta subunit protein and its application in a lateral flow assay for early diagnosis of leptospirosis.Medical microbiology and immunology · 2025Article
- Health assessment of non-native red-eared sliders (Trachemys scripta elegans) and their impact potential on native species.PloS one · 2025Article
- Leptospirosis Risk Assessment in Rodent Populations and Environmental Reservoirs in Humanitarian Aid Settings in Thailand.Microorganisms · 2024Article
- Leptospirosis outbreak in Ecuador in 2023: A pilot study for surveillance from a One Health perspective.One health (Amsterdam, Netherlands) · 2024Article
- The Challenge of Bacterial Strain Identification:Tropical medicine and infectious disease · 2024Article
72 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundLeptospirosis is an emerging infectious disease. The differential diagnosis of leptospirosis is difficult due to the varied and often "flu like" symptoms which may result in a missed or delayed diagnosis. There are over 230 known serovars in the genus Leptospira. Confirmatory serological diagnosis of leptospirosis is usually made using the microscopic agglutination test (MAT) which relies on the use of live cultures as the source of antigen, often performed using a panel of antigens representative of local serovars. Other techniques, such as the enzyme linked immunosorbent assay (ELISA) and slide agglutination test (SAT), can detect different classes of antibody but may be subject to false positive reactions and require confirmation of these results by the MAT.
methodsThe polymerase chain reaction (PCR) has been used to detect a large number of microorganisms, including those of clinical significance. The sensitivity of PCR often precludes the need for isolation and culture, thus making it ideal for the rapid detection of organisms involved in acute infections. We employed real-time (quantitative) PCR using TaqMan chemistry to detect leptospires in clinical and environmental samples. RESULTS AND
conclusionsThe PCR assay can be applied to either blood or urine samples and does not rely on the isolation and culture of the organism. Capability exists for automation and high throughput testing in a clinical laboratory. It is specific for Leptospira and may discriminate pathogenic and non-pathogenic species. The limit of detection is as low as two cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.