ArticleJournal of virology2002
Reassessing the role of region A in Pit1-mediated viral entry.
Article in Journal of virology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 36 citations in OpenAlex.
- A recent gibbon ape leukemia virus germline integration in a rodent from New Guinea.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- GALV-KoRV-related retroviruses in diverse Australian and African rodent species.Virus evolution · 2024Article
- Receptor-Loaded Virion Endangers GPCR Signaling: Mechanistic Exploration of SARS-CoV-2 Infections and Pharmacological Implications.International journal of molecular sciences · 2021Review
- Infectious KoRV-related retroviruses circulating in Australian bats.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- A mutant retroviral receptor restricts virus superinfection interference and productive infection.Retrovirology · 2012Article
- The receptors for gibbon ape leukemia virus and amphotropic murine leukemia virus are not downregulated in productively infected cells.Retrovirology · 2011Article
- Mapping of the minimal inorganic phosphate transporting unit of human PiT2 suggests a structure universal to PiT-related proteins from all kingdoms of life.BMC biochemistry · 2011Article
- Primate gammaretroviruses require an ancillary factor not required for murine gammaretroviruses to infect BHK cells.Journal of virology · 2011Article
- Naturally Occurring Polymorphisms of the Mouse Gammaretrovirus Receptors CAT-1 and XPR1 Alter Virus Tropism and Pathogenicity.Advances in virology · 2011Article
- The mouse "xenotropic" gammaretroviruses and their XPR1 receptor.Retrovirology · 2010Review
- New structural arrangement of the extracellular regions of the phosphate transporter SLC20A1, the receptor for gibbon ape leukemia virus.The Journal of biological chemistry · 2009Article
- Identification of a feline leukemia virus variant that can use THTR1, FLVCR1, and FLVCR2 for infection.Journal of virology · 2009Article
- Identification of two distinct structural regions in a human porcine endogenous retrovirus receptor, HuPAR2, contributing to function for viral entry.Retrovirology · 2009Article
- Comprehensive mapping of receptor-functioning domains in feline leukemia virus subgroup C receptor FLVCR1.Journal of virology · 2006Article
- Dissection of gammaretroviral receptor function by using type III phosphate transporters as models.Journal of virology · 2005Article
- The central half of Pit2 is not required for its function as a retroviral receptor.Journal of virology · 2004Article
- The SLC20 family of proteins: dual functions as sodium-phosphate cotransporters and viral receptors.Pflugers Archiv : European journal of physiology · 2004Review
- Identification of an extracellular domain within the human PiT2 receptor that is required for amphotropic murine leukemia virus binding.Journal of virology · 2004Article
- Identification of receptors for pig endogenous retrovirus.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Host range and receptor binding properties of vectors bearing feline leukemia virus subgroup B envelopes can be modulated by envelope sequences outside of the receptor binding domain.Journal of virology · 2002Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The mammalian gammaretroviruses gibbon ape leukemia virus (GALV) and feline leukemia virus subgroup B (FeLV-B) can use the same receptor, Pit1, to infect human cells. A highly polymorphic nine-residue sequence within Pit1, designated region A, has been proposed as the virus binding site, because mutations in this region abolish Pit1-mediated cellular infection by GALV and FeLV-B. However, a direct correlation between region A mutations deleterious for infection and loss of virus binding has not been established. We report that cells expressing a Pit1 protein harboring mutations in region A that abolish receptor function retain the ability to bind virus, indicating that Pit1 region A is not the virus binding site. Furthermore, we have now identified a second region in Pit1, comprising residues 232 to 260 (region B), that is required for both viral entry and virus binding. Epitope-tagged Pit1 proteins were used to demonstrate that mutations in region B result in improper orientation of Pit1 in the cell membrane. Compensatory mutations in region A can restore proper orientation and full receptor function to these region B mutants. Based on these results, we propose that region A of Pit1 confers competence for viral entry by influencing the topology of the authentic binding site in the membrane and hence its accessibility to a viral envelope protein. Based on glycosylation studies and results obtained by using N- and C-terminal epitope-tagged Pit1, region A and region B mutants, and the transmembrane helices predicted with the PHD PredictProtein algorithm, we propose a new Pit1 topology model.
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