ArticleJournal of virology2002
Zinc finger domain of murine leukemia virus nucleocapsid protein enhances the rate of viral DNA synthesis in vivo.
Article in Journal of virology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 39 citations in OpenAlex.
- Reverse Transcription of Retroviruses and LTR Retrotransposons.Microbiology spectrum · 2015Review
- HIV-1 reverse transcription.Cold Spring Harbor perspectives in medicine · 2012Review
- Moloney murine leukemia virus genomic RNA packaged in the absence of a full complement of wild type nucleocapsid protein.Virology · 2012Article
- MoMuLV and HIV-1 nucleocapsid proteins have a common role in genomic RNA packaging but different in late reverse transcription.PloS one · 2012Article
- Review
- The arginine clusters of the carboxy-terminal domain of the core protein of hepatitis B virus make pleiotropic contributions to genome replication.Journal of virology · 2011Article
- The remarkable frequency of human immunodeficiency virus type 1 genetic recombination.Microbiology and molecular biology reviews : MMBR · 2009Review
- Role of RNA chaperones in virus replication.Virus research · 2009Review
- Quantitative analysis of clinically relevant mutations occurring in lymphoid cells harboring gamma-retrovirus-encoded hsvtk suicide genes.Gene therapy · 2008Article
- Functional complementation of nucleocapsid and late domain PTAP mutants of human immunodeficiency virus type 1 during replication.Virology · 2008Article
- Nucleocapsid protein function in early infection processes.Virus research · 2008Review
- Ty3 nucleocapsid controls localization of particle assembly.Journal of virology · 2008Article
- Capsid is an important determinant for functional complementation of murine leukemia virus and spleen necrosis virus Gag proteins.Virology · 2007Article
- Effects of nucleic acid local structure and magnesium ions on minus-strand transfer mediated by the nucleic acid chaperone activity of HIV-1 nucleocapsid protein.Nucleic acids research · 2007Article
- Pausing during reverse transcription increases the rate of retroviral recombination.Journal of virology · 2006Article
- Cooperative effect of gag proteins p12 and capsid during early events of murine leukemia virus replication.Journal of virology · 2005Article
- Precise identification of endogenous proviruses of NFS/N mice participating in recombination with moloney ecotropic murine leukemia virus (MuLV) to generate polytropic MuLVs.Journal of virology · 2005Article
- Antiretroviral drug resistance mutations in human immunodeficiency virus type 1 reverse transcriptase increase template-switching frequency.Journal of virology · 2004Article
- Frequent dual initiation in human immunodeficiency virus-based vectors containing two primer-binding sites: a quantitative in vivo assay for function of initiation complexes.Journal of virology · 2004Article
- Rubella virus capsid protein modulates viral genome replication and virus infectivity.Journal of virology · 2004Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
In vitro studies have indicated that retroviral nucleocapsid (NC) protein facilitates both DNA synthesis by reverse transcriptase (RT) and annealing of the nascent DNA with acceptor template. Increasing the rate of DNA synthesis is expected to reduce the frequency of RT template switching, whereas annealing the nascent DNA with acceptor template promotes template switching. We performed a mutational analysis of the murine leukemia virus (MLV) NC zinc finger domain to study its effect on RT template switching in vivo and to explore the role of NC during reverse transcription. The effects of NC mutations on RT template switching were determined by using a previously described in vivo direct-repeat deletion assay. A trans-complementation assay was also developed in which replication-defective NC mutants were rescued by coexpression of replication-defective RT mutants that provided wild-type NC in trans. We found that mutations in the MLV NC zinc finger domain increased the frequency of template switching approximately twofold. When a predicted stem-loop RNA secondary structure was introduced into the template RNA, the template-switching frequency increased 5-fold for wild-type NC and further increased up to an additional 6-fold for NC zinc finger domain mutants, resulting in an overall increase of as much as 30-fold. Thus, wild-type NC increased the efficiency with which RT was able to reverse transcribe through regions of RNA secondary structure that might serve as RT pause sites. These results provide the first in vivo evidence that NC enhances the rate of DNA synthesis by RT in regions of the template possessing stable RNA secondary structure.
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