ArticleThe Biochemical journal2002
Biphasic translocation of Bax to mitochondria.
Article in The Biochemical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 82 citations in OpenAlex.
- Mitochondrial CaInternational journal of molecular sciences · 2022Review
- Heat stress induces apoptosis through disruption of dynamic mitochondrial networks in dairy cow mammary epithelial cells.In vitro cellular & developmental biology. Animal · 2020Article
- Completion of BAX recruitment correlates with mitochondrial fission during apoptosis.Scientific reports · 2019Article
- BAX to basics: How the BCL2 gene family controls the death of retinal ganglion cells.Progress in retinal and eye research · 2017Review
- Live-cell imaging to measure BAX recruitment kinetics to mitochondria during apoptosis.PloS one · 2017Article
- Cigarette smoke-induced mitochondrial fragmentation and dysfunction in human airway smooth muscle.American journal of physiology. Lung cellular and molecular physiology · 2014Article
- Deficiency of αB crystallin augments ER stress-induced apoptosis by enhancing mitochondrial dysfunction.Free radical biology & medicine · 2012Article
- VDAC1: from structure to cancer therapy.Frontiers in oncology · 2012Article
- Cytochrome c is rapidly reduced in the cytosol after mitochondrial outer membrane permeabilization.Apoptosis : an international journal on programmed cell death · 2010Article
- Active fragments from pro- and antiapoptotic BCL-2 proteins have distinct membrane behavior reflecting their functional divergence.PloS one · 2010Article
- Activation of Bax by joint action of tBid and mitochondrial outer membrane: Monte Carlo simulations.European biophysics journal : EBJ · 2009Article
- Mitochondrial fragmentation is involved in methamphetamine-induced cell death in rat hippocampal neural progenitor cells.PloS one · 2009Article
- Crosstalk signaling between mitochondrial Ca2+ and ROS.Frontiers in bioscience (Landmark edition) · 2009Review
- Differential patterns of peroxynitrite mediated apoptosis in proximal tubular epithelial cells following ATP depletion recovery.Apoptosis : an international journal on programmed cell death · 2008Article
- Evaluating cytochrome c diffusion in the intermembrane spaces of mitochondria during cytochrome c release.Journal of cell science · 2008Article
- Delivery of drugs and macromolecules to mitochondria.Advanced drug delivery reviews · 2007Review
- The N-terminal conformation of Bax regulates cell commitment to apoptosis.Cell death and differentiation · 2007Article
- Vital imaging of H9c2 myoblasts exposed to tert-butylhydroperoxide--characterization of morphological features of cell death.BMC cell biology · 2007Article
- Mitochondrial ion channels.Annual review of physiology · 2007Review
- Hexokinase II: cancer's double-edged sword acting as both facilitator and gatekeeper of malignancy when bound to mitochondria.Oncogene · 2006Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using green fluorescent protein-tagged Bax, we demonstrate that Bax is sequestered from the cytosol of cardiomyocytes in two distinct phases following the induction of apoptosis with staurosporine. In the first phase, lasting several hours, Bax removal from the cytosol was relatively small. In the second phase, Bax was very largely removed from the cytosol and sequestered into large aggregates associated with the mitochondria. To test which of the phases involved cytochrome c release, cells were transfected with a red fluorescent protein-cytochrome c fusion. The cytochrome c fusion protein was accumulated by mitochondria of healthy cells and was released by staurosporine in phase 1. When green fluorescent protein-Bax was immunoprecipitated from extracts of cells in phase 1 and phase 2, the voltage-dependent anion channel (mitochondrial outer membrane) and the adenine nucleotide translocase (mitochondrial inner membrane) were also precipitated. These data support a two-phase model of Bax translocation in which Bax targets the mitochondrial intermembrane contact sites and releases cytochrome c in the first phase, and is then packaged into large aggregates on mitochondria in the second.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.