ArticleProceedings of the National Academy of Sciences of the United States of America2002
A deletion-generator compound element allows deletion saturation analysis for genomewide phenotypic annotation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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27 citing papers in PubMed.
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- The disease-associated proteins Drosophila Nab2 and Ataxin-2 interact with shared RNAs and coregulate neuronal morphology.Genetics · 2022Article
- Pharmaco-Genetic Screen To Uncover Actin Regulators Targeted by Prostaglandins DuringG3 (Bethesda, Md.) · 2019Article
- Drosophila Lipophorin Receptors Recruit the Lipoprotein LTP to the Plasma Membrane to Mediate Lipid Uptake.PLoS genetics · 2015Article
- New gene evolution in the bonus-TIF1-γ/TRIM33 family impacted the architecture of the vertebrate dorsal-ventral patterning network.Molecular biology and evolution · 2014Article
- A developmental genetic analysis of the lysine demethylase KDM2 mutations in Drosophila melanogaster.Mechanisms of development · 2014Article
- Suicidal autointegration of sleeping beauty and piggyBac transposons in eukaryotic cells.PLoS genetics · 2014Article
- Neprilysins: an evolutionarily conserved family of metalloproteases that play important roles in reproduction in Drosophila.Genetics · 2014Article
- Regional modulation of a stochastically expressed factor determines photoreceptor subtypes in the Drosophila retina.Developmental cell · 2013Article
- The generation of chromosomal deletions to provide extensive coverage and subdivision of the Drosophila melanogaster genome.Genome biology · 2012Article
- Article
- Coordinated expression of cell death genes regulates neuroblast apoptosis.Development (Cambridge, England) · 2011Article
- Large-scale functional annotation and expanded implementations of the P{wHy} hybrid transposon in the Drosophila melanogaster genome.Genetics · 2009Article
- Construction and characterization of deletions with defined end points in Drosophila using P elements in trans.Genetics · 2009Article
- Macrotransposition and other complex chromosomal restructuring in maize by closely linked transposons in direct orientation.The Plant cell · 2008Article
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- Transposable elements in fish functional genomics: technical challenges and perspectives.Genome biology · 2007Review
- A gain-of-function screen identifying genes required for vein formation in the Drosophila melanogaster wing.Genetics · 2006Article
- Genetic interactions between Drosophila melanogaster menin and Jun/Fos.Developmental biology · 2006Article
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Abstract
With the available eukaryotic genome sequences, there are predictions of thousands of previously uncharacterized genes without known function or available mutational variant. Thus, there is an urgent need for efficient genetic tools for genomewide phenotypic analysis. Here we describe such a tool: a deletion-generator technology that exploits properties of a double transposable element to produce molecularly defined deletions at high density and with high efficiency. This double element, called P[wHy], is composed of a "deleter" element hobo, bracketed by two genetic markers and inserted into a "carrier" P element. We have used this P[wHy] element in Drosophila melanogaster to generate sets of nested deletions of sufficient coverage to discriminate among every transcription unit within 60 kb of the starting insertion site. Because these two types of mobile elements, carrier and deleter, can be found in other species, our strategy should be applicable to phenotypic analysis in a variety of model organisms.
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