ArticleThe Journal of physiology2002
Evidence against aquaporin-1-dependent CO2 permeability in lung and kidney.
Article in The Journal of physiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 82 citations in OpenAlex.
- Transport Characteristics of Aquaporins.Advances in experimental medicine and biology · 2023Article
- Proximal renal tubular acidosis with and without Fanconi syndrome.Kidney research and clinical practice · 2019Review
- Covalently Linking Oligomerization-Impaired GlpF Protomers Does Not Completely Re-establish Wild-Type Channel Activity.International journal of molecular sciences · 2019Article
- Mechanisms of Aquaporin-Facilitated Cancer Invasion and Metastasis.Frontiers in chemistry · 2018Review
- Renal acid-base regulation: new insights from animal models.Pflugers Archiv : European journal of physiology · 2015Review
- Increased water flux induced by an aquaporin-1/carbonic anhydrase II interaction.Molecular biology of the cell · 2015Article
- How does carbon dioxide permeate cell membranes? A discussion of concepts, results and methods.Frontiers in physiology · 2014Review
- Physiological roles of aquaporin-4 in brain.Physiological reviews · 2013Review
- In vivo NADH fluorescence imaging indicates effect of aquaporin-4 deletion on oxygen microdistribution in cortical spreading depression.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2013Article
- Molecular dynamics of water in the neighborhood of aquaporins.European biophysics journal : EBJ · 2013Review
- Uroplakins do not restrict CO2 transport through urothelium.The Journal of biological chemistry · 2012Article
- Sharpey-Schafer lecture: gas channels.Experimental physiology · 2010Article
- Interactions of multiple gas-transducing systems: hallmarks and uncertainties of CO, NO, and H2S gas biology.Antioxidants & redox signaling · 2010Review
- Exploring transmembrane diffusion pathways with molecular dynamics.Physiology (Bethesda, Md.) · 2010Review
- Nitric oxide conduction by the brain aquaporin AQP4.Proteins · 2010Article
- Cytoplasmic histidine kinase (HP0244)-regulated assembly of urease with UreI, a channel for urea and its metabolites, CO2, NH3, and NH4(+), is necessary for acid survival of Helicobacter pylori.Journal of bacteriology · 2010Article
- Plant aquaporin selectivity: where transport assays, computer simulations and physiology meet.Cellular and molecular life sciences : CMLS · 2009Review
- Aquaporins: translating bench research to human disease.The Journal of experimental biology · 2009Review
- High-resolution x-ray structure of human aquaporin 5.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Mammalian aquaporins: diverse physiological roles and potential clinical significance.Expert reviews in molecular medicine · 2008Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
AQP1-dependent CO2 transport has been suggested from the increased CO2 permeability in Xenopus oocytes expressing AQP1. Potential implications of this finding include AQP1-facilitated CO2 exchange in mammalian lung and HCO3-/CO2 transport in kidney proximal tubule. We reported previously that: (a) CO2 permeability in erythrocytes was not affected by AQP1 deletion, (b) CO2 permeability in liposomes was not affected by AQP1 reconstitution despite a 100-fold increased water permeability, and (c) CO2 blow-off by the lung in living mice was not impaired by AQP1 deletion. We extend these observations by direct measurement of CO2 permeabilities in lung and kidney. CO2 transport across the air-space-capillary barrier in isolated perfused lungs was measured from changes in air-space fluid pH in response to addition/removal of HCO3-/CO2 from the pulmonary artery perfusate. The pH was measured by pleural surface fluorescence of a pH indicator (BCECF-dextran) in the air-space fluid. Air-space fluid pH equilibrated rapidly (t(1/2) approximately 6 s) in response to addition/removal of HCO3-/CO2. However, the kinetics of pH change was not different in lungs of mice lacking AQP1, AQP5 or AQP1/AQP5 together, despite an up to 30-fold reduction in water permeability. CO2 transport across BCECF-loaded apical membrane vesicles from kidney proximal tubule was measured from the kinetics of intravesicular acidification in response to rapid mixing with a HCO3-/CO2 solution. Vesicles rapidly acidified (t(1/2) approximately 10 ms) in response to HCO3-/CO2 addition. However the acidification rate was not different in kidney vesicles from AQP1-null mice despite a 20-fold reduction in water permeability. The results provide direct evidence against physiologically significant transport of CO2 by AQP1 in mammalian lung and kidney.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.