ArticleThe Biochemical journal2002
Genistein inhibits CCAAT/enhancer-binding protein beta (C/EBPbeta) activity and 3T3-L1 adipogenesis by increasing C/EBP homologous protein expression.
Article in The Biochemical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed, 106 citations in OpenAlex.
- Natural Bioactive Compounds Targeting FABP4 in Adipogenesis and Obesity: Evidence from In Vitro and In Vivo Studies.International journal of molecular sciences · 2026Review
- A novel lncRNA enhances autophagy to suppress extracellular matrix via modulating TP53INP1 in human trabecular meshwork cells under oxidative stress.Scientific reports · 2025Article
- Natural bioactive compounds and their mechanisms of action in the management of obesity: a narrative review.Frontiers in nutrition · 2025Review
- Crosstalk between adipogenesis and aging: role of polyphenols in combating adipogenic-associated aging.Immunity & ageing : I & A · 2024Review
- Natural products as novel anti-obesity agents: insights into mechanisms of action and potential for therapeutic management.Frontiers in pharmacology · 2023Review
- Article
- KRAS Affects Adipogenic Differentiation by Regulating Autophagy and MAPK Activation in 3T3-L1 and C2C12 Cells.International journal of molecular sciences · 2021Article
- Combined Beneficial Effect of Genistein and Atorvastatin on Adipogenesis in 3T3-L1 Adipocytes.Biomolecules · 2021Article
- Adipogenesis as a Potential Anti-Obesity Target: A Review of Pharmacological Treatment and Natural Products.Diabetes, metabolic syndrome and obesity : targets and therapy · 2021Review
- Crocetin Isolated from the Natural Food Colorant Saffron Reduces Intracellular Fat in 3T3-L1 Adipocytes.Foods (Basel, Switzerland) · 2020Article
- Participation of MicroRNAs in the Treatment of Cancer with Phytochemicals.Molecules (Basel, Switzerland) · 2020Review
- Genistin: A Novel Potent Anti-Adipogenic and Anti-Lipogenic Agent.Molecules (Basel, Switzerland) · 2020Article
- In vitro biological evaluation of anti-diabetic activity of organic-inorganic hybrid gold nanoparticles.IET nanobiotechnology · 2019Article
- Natural Products and Obesity: A Focus on the Regulation of Mitotic Clonal Expansion during Adipogenesis.Molecules (Basel, Switzerland) · 2019Review
- Anti-inflammatory activity of elicited soybean (Central-European journal of immunology · 2019Article
- Article
- Cellular models for the evaluation of the antiobesity effect of selected phytochemicals from food and herbs.Journal of food and drug analysis · 2017Review
- Potential of Natural Products in the Inhibition of Adipogenesis through Regulation of PPARγ Expression and/or Its Transcriptional Activity.Molecules (Basel, Switzerland) · 2016Review
- Molecular Regulation of Adipogenesis and Potential Anti-Adipogenic Bioactive Molecules.International journal of molecular sciences · 2016Review
- Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The tyrosine kinase inhibitor genistein inhibits 3T3-L1 adipogenesis when present during the first 72 h of differentiation. In this report, we investigated the underlying mechanisms involved in the anti-adipogenic effects of genistein. We found that genistein blocked the DNA binding and transcriptional activity of CCAAT/enhancer-binding protein beta (C/EBPbeta) during differentiation by promoting the expression of C/EBP homologous protein, a dominant-negative member of the C/EBP family. Loss of C/EBPbeta activity was manifested as a loss of differentiation-induced C/EBPalpha and peroxisome-proliferator-activated receptor gamma protein expression and a dramatic reduction in lipid accumulation. Further, we documented for the first time that C/EBPbeta was tyrosine-phosphorylated in vivo during differentiation and in vitro by activated epidermal growth factor receptor. Genistein inhibited both of these events. Collectively, these results indicate that genistein blocks adipogenesis and C/EBPbeta activity by increasing the level of C/EBP homologous protein and possibly by inhibiting the tyrosine phosphorylation of C/EBPbeta.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.