ArticleThe Journal of cell biology2002
Interference with the cytoplasmic tail of gp210 disrupts "close apposition" of nuclear membranes and blocks nuclear pore dilation.
Article in The Journal of cell biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 58 citations in OpenAlex.
- Molecular architecture of the luminal ring of the Xenopus laevis nuclear pore complex.Cell research · 2020Article
- Mice Deficient in Nucleoporin Nup210 Develop Peripheral T Cell Alterations.Frontiers in immunology · 2018Article
- Inner/Outer nuclear membrane fusion in nuclear pore assembly: biochemical demonstration and molecular analysis.Molecular biology of the cell · 2010Article
- Transportin mediates nuclear entry of DNA in vertebrate systems.Traffic (Copenhagen, Denmark) · 2009Article
- Capture of AT-rich chromatin by ELYS recruits POM121 and NDC1 to initiate nuclear pore assembly.Molecular biology of the cell · 2008Article
- Atomic force microscopy visualises a hydrophobic meshwork in the central channel of the nuclear pore.Pflugers Archiv : European journal of physiology · 2008Article
- Centrin 2 localizes to the vertebrate nuclear pore and plays a role in mRNA and protein export.Molecular and cellular biology · 2008Article
- Biology and biophysics of the nuclear pore complex and its components.International review of cell and molecular biology · 2008Review
- Topology of yeast Ndc1p: predictions for the human NDC1/NET3 homologue.The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology · 2006Article
- The role of the integral membrane nucleoporins Ndc1p and Pom152p in nuclear pore complex assembly and function.The Journal of cell biology · 2006Article
- Nuclear pore complex function in Saccharomyces cerevisiae is influenced by glycosylation of the transmembrane nucleoporin Pom152p.Genetics · 2005Article
- Despite WT1 binding sites in the promoter region of human and mouse nucleoporin glycoprotein 210, WT1 does not influence expression of GP210.Journal of negative results in biomedicine · 2004Article
- A novel allele of Saccharomyces cerevisiae NDC1 reveals a potential role for the spindle pole body component Ndc1p in nuclear pore assembly.Eukaryotic cell · 2004Article
- Importin beta negatively regulates nuclear membrane fusion and nuclear pore complex assembly.Molecular biology of the cell · 2003Article
- Nuclear pore protein gp210 is essential for viability in HeLa cells and Caenorhabditis elegans.Molecular biology of the cell · 2003Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
We tested the hypothesis that gp210, an integral membrane protein of nuclear pore complexes (NPCs), mediates nuclear pore formation. Gp210 has a large lumenal domain and small COOH-terminal tail exposed to the cytoplasm. We studied the exposed tail. We added recombinant tail polypeptides to Xenopus nuclear assembly extracts, or inhibited endogenous gp210 tails using anti-tail antibodies. Both strategies had no effect on the formation of fused flattened nuclear membranes, but blocked NPC assembly and nuclear growth. Inhibited nuclei accumulated gp210 and some nucleoporin p62, but failed to incorporate nup214/CAN, nup153, or nup98 and were defective for nuclear import of lamin B3. Scanning and transmission EM revealed a lack of "closely apposed" inner and outer membranes, and the accumulation of novel arrested structures including "mini-pores." We conclude that gp210 has early roles in nuclear pore formation, and that pore dilation is mediated by gp210 and its tail-binding partner(s). We propose that membrane fusion and pore dilation are coupled, acting as a mechanism to control nuclear pore size.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.