ArticleThe EMBO journal2002
Cryo-EM reveals an active role for aminoacyl-tRNA in the accommodation process.
Article in The EMBO journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 124 papers.
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Who cites it
124 citing papers in PubMed.
- HflX-mediated drug resistance through ribosome splitting and rRNA disordering in mycobacteria.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Drug resistance through ribosome splitting and rRNA disordering in mycobacteria.bioRxiv : the preprint server for biology · 2024Article
- Geometric alignment of aminoacyl-tRNA relative to catalytic centers of the ribosome underpins accurate mRNA decoding.Nature communications · 2023Article
- Exploring the Structural Variability of Dynamic Biological Complexes by Single-Particle Cryo-Electron Microscopy.Micromachines · 2022Review
- CryoEM Reveals the Complexity and Diversity of ATP Synthases.Frontiers in microbiology · 2022Review
- Ribosome quality control antagonizes the activation of the integrated stress response on colliding ribosomes.Molecular cell · 2021Article
- Structural Basis for Enzymatic Off-Loading of Hybrid Polyketides by Dieckmann Condensation.ACS chemical biology · 2020Article
- tRNA Dissociation from EF-Tu after GTP Hydrolysis: Primary Steps and Antibiotic Inhibition.Biophysical journal · 2020Article
- Insights into the base-pairing preferences of 8-oxoguanosine on the ribosome.Nucleic acids research · 2019Article
- Analysis of discrete local variability and structural covariance in macromolecular assemblies using Cryo-EM and focused classification.Ultramicroscopy · 2019Article
- Joachim Frank's Binding with the Ribosome.Structure (London, England : 1993) · 2019Review
- Ribosomal ambiguity (ram) mutations promote the open (off) to closed (on) transition and thereby increase miscoding.Nucleic acids research · 2019Article
- Dynamic analysis of ribosome by a movie made from many three-dimensional electron-microscopy density maps.Biophysics and physicobiology · 2019Article
- Cryo-EM shows stages of initial codon selection on the ribosome by aa-tRNA in ternary complex with GTP and the GTPase-deficient EF-TuH84A.Nucleic acids research · 2018Article
- Accuracy of genetic code translation and its orthogonal corruption by aminoglycosides and Mg2+ ions.Nucleic acids research · 2018Article
- Elfamycins: inhibitors of elongation factor-Tu.Molecular microbiology · 2017Review
- Single-Molecule Photoactivation FRET: A General and Easy-To-Implement Approach To Break the Concentration Barrier.Angewandte Chemie (International ed. in English) · 2017Article
- Article
- Small methyltransferase RlmH assembles a composite active site to methylate a ribosomal pseudouridine.Scientific reports · 2017Article
- The translation elongation cycle-capturing multiple states by cryo-electron microscopy.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Review
64 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors.
Funding
Abstract
During the elongation cycle of protein biosynthesis, the specific amino acid coded for by the mRNA is delivered by a complex that is comprised of the cognate aminoacyl-tRNA, elongation factor Tu and GTP. As this ternary complex binds to the ribosome, the anticodon end of the tRNA reaches the decoding center in the 30S subunit. Here we present the cryo- electron microscopy (EM) study of an Escherichia coli 70S ribosome-bound ternary complex stalled with an antibiotic, kirromycin. In the cryo-EM map the anticodon arm of the tRNA presents a new conformation that appears to facilitate the initial codon-anticodon interaction. Furthermore, the elbow region of the tRNA is seen to contact the GTPase-associated center on the 50S subunit of the ribosome, suggesting an active role of the tRNA in the transmission of the signal prompting the GTP hydrolysis upon codon recognition.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.