ArticleNature biotechnology2002
Genome-wide internal tagging of bacterial exported proteins.
Article in Nature biotechnology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 37 citations in OpenAlex.
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- Identification of genes involved in exoprotein release using a high-throughput exoproteome screening assay in Yersinia entomophaga.PloS one · 2022Article
- Destabilization of the Tumor-Inducing Plasmid from an Octopine-TypeG3 (Bethesda, Md.) · 2019Article
- Comprehensive Arrayed Transposon Mutant Library of Klebsiella pneumoniae Outbreak Strain KPNIH1.Journal of bacteriology · 2017Article
- Role of the Transporter-Like Sensor Kinase CbrA in Histidine Uptake and Signal Transduction.Journal of bacteriology · 2015Article
- NLS-tagging: an alternative strategy to tag nuclear proteins.Nucleic acids research · 2014Article
- Role for ferredoxin:NAD(P)H oxidoreductase (FprA) in sulfate assimilation and siderophore biosynthesis in Pseudomonads.Journal of bacteriology · 2013Article
- Membrane proteases and aminoglycoside antibiotic resistance.Journal of bacteriology · 2011Article
- MexEF-OprN efflux pump exports the Pseudomonas quinolone signal (PQS) precursor HHQ (4-hydroxy-2-heptylquinoline).PloS one · 2011Article
- Article
- Genetic toggling of alkaline phosphatase folding reveals signal peptides for all major modes of transport across the inner membrane of bacteria.The Journal of biological chemistry · 2008Article
- The potential of desferrioxamine-gallium as an anti-Pseudomonas therapeutic agent.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Targeted mutagenesis of Burkholderia thailandensis and Burkholderia pseudomallei through natural transformation of PCR fragments.Applied and environmental microbiology · 2008Article
- Identification of biofilm-associated cluster (bac) in Pseudomonas aeruginosa involved in biofilm formation and virulence.PloS one · 2008Article
- Dual involvement of CbrAB and NtrBC in the regulation of histidine utilization in Pseudomonas fluorescens SBW25.Genetics · 2008Article
- Mutational activation of niche-specific genes provides insight into regulatory networks and bacterial function in a complex environment.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Iron and Pseudomonas aeruginosa biofilm formation.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Comprehensive transposon mutant library of Pseudomonas aeruginosa.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Novel methylotrophy genes of Methylobacterium extorquens AM1 identified by using transposon mutagenesis including a putative dihydromethanopterin reductase.Journal of bacteriology · 2003Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
As a result of the explosive growth of bacterial genomic and postgenomic information, there is a pressing need for efficient, inexpensive strategies for characterizing the in vivo behavior and function of newly identified gene products. We describe here an internal tagging procedure, based on transposon technology, to facilitate the analysis of membrane-bound and secreted proteins in Gram-negative bacteria. The technique is based on a broad host range transposon (ISphoA/hah), which may be used to generate both alkaline phosphatase (AP) gene fusions and 63-codon in-frame insertions in the genome. The 63-codon insertion encodes an influenza hemagglutinin epitope and a hexahistidine sequence, permitting sensitive detection and metal affinity purification of tagged proteins. For each gene targeted, it is thus possible to monitor the disruption of phenotype (using the transposon insertion), the gene's transcription and translation (using the AP reporter activity), and the behavior of the unfused protein (using the internal tag). Studies on a sequence-defined collection of Escherichia coli strains generated using the transposon showed that the synthesis and subcellular localization of tagged proteins could be readily monitored. The use of ISphoA/hah should provide a cost-effective approach for genome-wide in vivo studies of the behavior of exported proteins in a number of bacterial species.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.