ArticlePlant molecular biology2002
Regulation of the high-affinity ammonium transporter (BnAMT1;2) in the leaves of Brassica napus by nitrogen status.
Article in Plant molecular biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 59 citations in OpenAlex.
- Origin and Evolution of Ammonium Transporters (AMTs) in Plants and Systematic Expression Studies inPlants (Basel, Switzerland) · 2026Article
- The Identification ofPlants (Basel, Switzerland) · 2024Article
- Genome-wide identification and expression analysis of ammonium transporter 1 (AMT1) gene family in cassava (3 Biotech · 2022Article
- Function and Regulation of Ammonium Transporters in Plants.International journal of molecular sciences · 2020Review
- Article
- Ammonium Transporter (Frontiers in plant science · 2019Article
- The AMT1 family genes from Malus robusta display differential transcription features and ammonium transport abilities.Molecular biology reports · 2017Article
- Wheat Ammonium Transporter (AMT) Gene Family: Diversity and Possible Role in Host-Pathogen Interaction with Stem Rust.Frontiers in plant science · 2017Article
- Influence of different host associations on glutamine synthetase activity and ammonium transporter inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2016Article
- Overexpressing of OsAMT1-3, a High Affinity Ammonium Transporter Gene, Modifies Rice Growth and Carbon-Nitrogen Metabolic Status.International journal of molecular sciences · 2015Article
- Urea retranslocation from senescing Arabidopsis leaves is promoted by DUR3-mediated urea retrieval from leaf apoplast.The Plant journal : for cell and molecular biology · 2015Article
- Influence of different nitrogen inputs on the members of ammonium transporter and glutamine synthetase genes in two rice genotypes having differential responsiveness to nitrogen.Molecular biology reports · 2012Article
- Understanding the differential nitrogen sensing mechanism in rice genotypes through expression analysis of high and low affinity ammonium transporter genes.Molecular biology reports · 2012Article
- The cloning and characterization of two ammonium transporters in the salt-resistant green alga, Dunaliella viridis.Molecular biology reports · 2011Article
- Responses of rice cultivars with different nitrogen use efficiency to partial nitrate nutrition.Annals of botany · 2007Article
- Ammonium transporter genes in Chlamydomonas: the nitrate-specific regulatory gene Nit2 is involved in Amt1;1 expression.Plant molecular biology · 2004Article
- Photorespiratory NH(4)(+) production in leaves of wild-type and glutamine synthetase 2 antisense oilseed rape.Plant physiology · 2002Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Substantial concentrations of NH4+ are found in the apoplast of the leaves of Brassica napus. Physiological studies on isolated mesophyll protoplasts with 15NH4+ revealed the presence of a high-affinity ammonium transporter that shared physiological similarity to the high-affinity NH4+ transporters in Arabidopsis thaliana (AtAMT1;3). PCR techniques were used to isolate a full-length clone of a B. napus homologue of AMT1 from shoot mRNA which showed 97% similarity to AtAMT1;3. The full-length cDNA when cloned into the yeast expression vector pFL61 was able to complement a yeast mutant unable to grow on media with NH4+ as the sole nitrogen source. Regulatory studies with detached leaves revealed a stimulation of both NH4+ uptake and expression of mRNA when the leaves were supplied with increasing concentrations of NH4+. Withdrawal of NH4+ supply for up to 96 h had little effect on mRNA expression or NH4+ uptake; however, plants grown continuously at high NH4+ levels exhibited decreased mRNA expression. BnAMT1;2 mRNA expression was highest when NH4+ was supplied directly to the leaf and lowest when either glutamine or glutamate was supplied to the leaves, which directly paralleled chloroplastic glutamine synthetase (GS2) activity in the same leaves. These results provide tentative evidence that BnAMT1;2 may be regulated by similar mechanisms to GS2 in leaves.
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