ArticleClinical & experimental metastasis2002
Optically imageable metastatic model of human breast cancer.
Article in Clinical & experimental metastasis, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 48 citations in OpenAlex.
- State of the Art Modelling of the Breast Cancer Metastatic Microenvironment: Where Are We?Journal of mammary gland biology and neoplasia · 2024Review
- Rare-Earth-Metal (NdPharmaceutics · 2022Article
- Preclinical Breast Cancer Models to Investigate Metabolic Priming by Methionine Restriction.Methods in molecular biology (Clifton, N.J.) · 2019Article
- Murine model of hepatic breast cancer.Biochemistry and biophysics reports · 2016Article
- Role of Fluorophore Charge on the In Vivo Optical Imaging Properties of Near-Infrared Cyanine Dye/Monoclonal Antibody Conjugates.Bioconjugate chemistry · 2016Article
- An improved syngeneic orthotopic murine model of human breast cancer progression.Breast cancer research and treatment · 2014Article
- Tumor-targeting Salmonella typhimurium A1-R prevents experimental human breast cancer bone metastasis in nude mice.Oncotarget · 2014Article
- Characterization of metastasis formation and virotherapy in the human C33A cervical cancer model.PloS one · 2014Article
- In vivo fluorescence imaging reveals the promotion of mammary tumorigenesis by mesenchymal stromal cells.PloS one · 2013Article
- Animal models and molecular imaging tools to investigate lymph node metastases.Journal of molecular medicine (Berlin, Germany) · 2011Review
- MicroRNA expression characterizes oligometastasis(es).PloS one · 2011Article
- Real-time imaging of tumor progression in a fluorescent orthotopic mouse model of thyroid cancer.Anticancer research · 2010Article
- Gain of a 500-fold sensitivity on an intravital MR contrast agent based on an endohedral gadolinium-cluster-fullerene-conjugate: a new chance in cancer diagnostics.International journal of medical sciences · 2010Article
- Human breast cancer cell lines co-express neuronal, epithelial, and melanocytic differentiation markers in vitro and in vivo.PloS one · 2010Article
- Article
- Comparison of mouse mammary gland imaging techniques and applications: reflectance confocal microscopy, GFP imaging, and ultrasound.BMC cancer · 2008Article
- A generic research paradigm for identification and validation of early molecular diagnostics and new therapeutics in common disorders.Molecular diagnosis & therapy · 2007Review
- Noninvasive optical tracking of red fluorescent protein-expressing cancer cells in a model of metastatic breast cancer.Neoplasia (New York, N.Y.) · 2006Article
- Gene transfer and expression of enhanced green fluorescent protein in variant HT-29c cells.World journal of gastroenterology · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
We report an optically imageable orthotopic metastatic nude mouse model of the human breast cancer MDA-MB-435 expressing green fluorescent protein (GFP). We demonstrate fluorescent imaging of primary and metastatic growth in live tissue and in intact animals. Fragments of tumor tissue expressing GFP were sutured into the pocket in the right second mammary gland. Tumor tissue was strongly fluorescent, enabling whole-body imaging of tumor growth by week 5. Neovascularization of the primary tumor was also visualized by whole-body imaging by contrast of the vessels to the fluorescent tumor. At autopsy, the MDA-MB-435-GFP was found to have metastasized to various organs, including the lung in 55% of the animals, the lymph nodes in 15% of the animals including axillary nodes, and the liver in 10% of the animals. These metastases could be visualized in fresh tissue by fluorescent imaging. Detailed fluorescence analysis visualized extensive metastasis in the thoracic cavity and the lymphatic system. Large metastatic nodules in the lung involved most of the pulmonary parenchyma in all lobes. Lymph node metastasis was found mainly in the axillary area. In the liver, fluorescent macroscopic metastatic nodules were found under the capsule. The metastatic pattern in the model thus reflected clinical metastatic breast cancer and provides a powerful model for drug discovery for this disease.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.