ArticleJournal of clinical microbiology2002
Application of RNA polymerase beta-subunit gene (rpoB) sequences for the molecular differentiation of Legionella species.
Article in Journal of clinical microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 91 citations in OpenAlex.
- Comparative single-cell genomics of two uncultivatedmSphere · 2025Article
- Identification of Legionella by MALDI Biotyper through three preparation methods and an in-house library comparing phylogenetic and hierarchical cluster results.Scientific reports · 2025Article
- Characterization of a Novel Species ofPathogens (Basel, Switzerland) · 2024Article
- Draft Genome Sequence ofMicrobiology resource announcements · 2022Article
- Combining Traditional and Molecular Techniques Supports the Discovery of a NovelFrontiers in microbiology · 2022Article
- New Insight regardingMicrobiology spectrum · 2021Article
- A robust phylogenetic framework for members of the order Legionellales and its main genera (Legionella, Aquicella, Coxiella and Rickettsiella) based on phylogenomic analyses and identification of molecular markers demarcating different clades.Antonie van Leeuwenhoek · 2021Article
- Draft Genome Sequences ofMicrobiology resource announcements · 2021Article
- Structural and Functional Investigations of the Effector Protein LpiR1 from Legionella pneumophila.The Journal of biological chemistry · 2016Article
- Genomic analysis of 38 Legionella species identifies large and diverse effector repertoires.Nature genetics · 2016Article
- Biogeography of Nocardiopsis strains from hypersaline environments of Yunnan and Xinjiang Provinces, western China.Scientific reports · 2015Article
- Comprehensive genomic characterization of campylobacter genus reveals some underlying mechanisms for its genomic diversification.PloS one · 2013Article
- Application of multilocus sequence analysis (MLSA) for accurate identification of Legionella spp. Isolated from municipal fountains in Chengdu, China, based on 16S rRNA, mip, and rpoB genes.Journal of microbiology (Seoul, Korea) · 2012Article
- Extensive recombination events and horizontal gene transfer shaped the Legionella pneumophila genomes.BMC genomics · 2011Article
- Development of Porphyromonas gingivalis-specific quantitative real-time PCR primers based on the nucleotide sequence of rpoB.Journal of microbiology (Seoul, Korea) · 2011Article
- Distribution of Legionella species from environmental water sources of public facilities and genetic diversity of L. pneumophila serogroup 1 in South Korea.Applied and environmental microbiology · 2010Article
- Legionella feeleii serotype 2 pneumonia in a man with chronic lymphocytic leukemia: a challenging diagnosis.Journal of clinical microbiology · 2010Article
- Application of rpoB and zinc protease gene for use in molecular discrimination of Fusobacterium nucleatum subspecies.Journal of clinical microbiology · 2010Article
- Characterization of Exiguobacterium isolates from the Siberian permafrost. Description of Exiguobacterium sibiricum sp. nov.Extremophiles : life under extreme conditions · 2006Article
- New species of Bordetella, Bordetella ansorpii sp. nov., isolated from the purulent exudate of an epidermal cyst.Journal of clinical microbiology · 2005Article
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The nucleotide sequences of the partial rpoB gene were determined from 38 Legionella species, including 15 serogroups of Legionella pneumophila. These sequences were then used to infer the phylogenetic relationships among the Legionella species in order to establish a molecular differentiation method appropriate for them. The sequences (300 bp) and the phylogenetic tree of rpoB were compared to those from analyses using 16S rRNA gene and mip sequences. The trees inferred from these three gene sequences revealed significant differences. This sequence incongruence between the rpoB tree and the other trees might have originated from the high frequency of synonymous base substitutions and/or from horizontal gene transfer among the Legionella species. The nucleotide variation of rpoB enabled more evident differentiation among the Legionella species than was achievable by the 16S rRNA gene and even by mip in some cases. Two subspecies of L. pneumophila (L. pneumophila subsp. pneumophila and subsp. fraseri) were clearly distinguished by rpoB but not by 16S rRNA gene and mip analysis. One hundred and five strains isolated from patient tissues and environments in Korea and Japan could be identified by comparison of rpoB sequence similarity and phylogenetic trees. These results suggest that the partial sequences of rpoB determined in this study might be applicable to the molecular differentiation of Legionella species.
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