ArticleJournal of clinical microbiology2002
Seminested PCR for diagnosis of candidemia: comparison with culture, antigen detection, and biochemical methods for species identification.
Article in Journal of clinical microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers, 1 of them a synthesis that pooled it.
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Who cites it
49 citing papers in PubMed, 1 synthesis or guideline pooled it, 216 citations in OpenAlex.
- PCR diagnosis of invasive candidiasis: systematic review and meta-analysis.Journal of clinical microbiology · 2011Pooled it
- Molecular quantification and differentiation of Candida species in biological specimens of patients with liver cirrhosis.PloS one · 2018Trial
- Review
- Molecular detection and genetic analysis ofVeterinary research forum : an international quarterly journal · 2024Article
- Comparison between Polymerase Chain Reaction and Blood Culture for Diagnosis of Neonatal Sepsis.Archives of Razi Institute · 2023Article
- Article
- Performance of Multiplex PCR and β-1,3-D-Glucan Testing for the Diagnosis of Candidemia.Journal of fungi (Basel, Switzerland) · 2022Article
- Utility of nested polymerase chain reaction for fungus in detecting clinically suspected patients of invasive fungal infections and its clinical correlation and comparison with fungal culture.Journal of family medicine and primary care · 2020Article
- Identification of Mycoses in Developing Countries.Journal of fungi (Basel, Switzerland) · 2019Review
- Relationship Between the Quantity of Oral Candida and Systemic Condition/Diseases of the Host: Oral Candida Increases with Advancing Age and Anemia.Mycopathologia · 2019Article
- Article
- Candida parapsilosis and Candida tropicalis infections in an Okhotsk snailfish (Liparis ochotensis).The Journal of veterinary medical science · 2018Article
- Prevalence of candidemia and associated candida subtypes following severe sepsis in non-neutropenic critically ill patients.Acta bio-medica : Atenei Parmensis · 2018Article
- Comparison of CHROMagar, polymerase chain reaction-restriction fragment length polymorphism, and polymerase chain reaction-fragment size for the identification of Candida species.Current medical mycology · 2017Article
- Microbial abundance on the eggs of a passerine bird and related fitness consequences between urban and rural habitats.PloS one · 2017Article
- Article
- Rapid identification of fungi in culture-negative clinical blood and respiratory samples by DNA sequence analyses.BMC research notes · 2016Article
- Beyond Blood Culture and Gram Stain Analysis: A Review of Molecular Techniques for the Early Detection of Bacteremia in Surgical Patients.Surgical infections · 2016Review
- Intestinal Microbial Metabolites Are Linked to Severity of Myocardial Infarction in Rats.PloS one · 2016Article
- Rapid identification of medically important Candida isolates using high resolution melting analysis.PloS one · 2015Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The rapid detection and identification of Candida species in clinical laboratories are extremely important for the management of patients with hematogenous candidiasis. The presently available culture and biochemical methods for detection and species identification of Candida are time-consuming and lack the required sensitivity and specificity. In this study, we have established a seminested PCR (snPCR) using universal and species-specific primers for detection of Candida species in serum specimens. The universal outer primers amplified the 3' end of 5.8S ribosomal DNA (rDNA) and the 5' end of 28S rDNA, including the internally transcribed spacer 2 (ITS2), generating 350- to 410-bp fragments from the four commonly encountered Candida species, viz., C. albicans, C. tropicalis, C. glabrata, and C. parapsilosis. The species-specific primers, complementary to unique sequences within the ITS2 of each test species, amplified species-specific DNA in the reamplification step of the snPCR. The sensitivity of Candida detection by snPCR in spiked serum specimens was close to 1 organism/ml. Evaluation of snPCR for specific identification of Candida species with 76 clinical Candida isolates showed 99% concordant results with the Vitek and/or ID32C yeast identification system. Further evaluation of snPCR for detection of Candida species in sera from culture-proven (n = 12), suspected (n = 16), and superficially colonized (n = 10) patients and healthy subjects (n = 12) showed that snPCR results were consistently negative with sera from healthy individuals and colonized patients. In culture-proven candidemia patients, the snPCR results were in full agreement with blood culture results with respect to both positivity and species identity. In addition, snPCR detected candidemia due to two Candida species in five patients, compared to three by blood culture. In the category of suspected candidemia with negative blood cultures for Candida, nine patients (56%) were positive by snPCR; two of them had dual infection with C. albicans and either C. tropicalis or C. glabrata. In conclusion, the snPCR developed in this study is specific and more sensitive than culture for the detection of Candida species in serum specimens. Moreover, the improved detection of cases of candidemia caused by more than one Candida species is an additional advantage.
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