ArticleJournal of clinical microbiology2002
Real-time PCR for quantification of human herpesvirus 6 DNA from lymph nodes and saliva.
Article in Journal of clinical microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed.
- Comprehensive polymerase chain reaction assay for detection of pathogenic DNA in lymphoproliferative disorders of the ocular adnexa.Scientific reports · 2016Trial
- Surveillance of active human herpesvirus 6 infection in chinese patients after hematopoietic stem cell transplantation with 3 different methods.International journal of hematology · 2006Trial
- Effect of prophylactic valacyclovir on the presence of human herpesvirus DNA in saliva of healthy individuals after dental treatment.Journal of clinical microbiology · 2005Trial
- Analysis Of Salivary Herpesviruses Reveals Associations Between HHV-6 And Long COVID Severity.medRxiv : the preprint server for health sciences · 2026Article
- Association betweenFrontiers in microbiology · 2026Article
- Symptomatic central nervous system tuberculosis and human herpesvirus-6 coinfection with associated hydrocephalus managed with endoscopic third ventriculostomy: A case report and review of human herpesvirus-6 neuropathology.Surgical neurology international · 2024Article
- Nectin-2 Acts as a Viral Entry Mediated Molecule That Binds to Human Herpesvirus 6B Glycoprotein B.Viruses · 2022Article
- Human herpes virus type-6 is associated with central nervous system infections in children in Sudan.African journal of laboratory medicine · 2022Article
- Differential Impacts of HHV-6A versus HHV-6B Infection in Differentiated Human Neural Stem Cells.Frontiers in immunology · 2022Article
- Chronic Alcoholism and HHV-6 Infection Synergistically Promote Neuroinflammatory Microglial Phenotypes in theBiomedicines · 2021Article
- Effect ofMicroorganisms · 2021Article
- Molecular Detection of Epstein-Barr Virus, Human Herpes Virus 6, Cytomegalovirus, and Hepatitis B Virus in Patients with Multiple Sclerosis.Middle East journal of digestive diseases · 2020Article
- Human Herpesvirus 6 and Malignancy: A Review.Frontiers in oncology · 2018Review
- Lymphoproliferative Syndromes Associated with Human Herpesvirus-6A and Human Herpesvirus-6B.Mediterranean journal of hematology and infectious diseases · 2018Review
- Successful treatment and FDG-PET/CT monitoring of HHV-6 encephalitis in a non-neutropenic patient: case report and literature review.Journal of neurovirology · 2017Article
- Virome characterisation from Guthrie cards in children who later developed acute lymphoblastic leukaemia.British journal of cancer · 2016Article
- Association of HHV-6 with Hodgkin and non Hodgkin lymphoma.Iranian journal of microbiology · 2016Article
- Optimisation of a quantitative polymerase chain reaction-based strategy for the detection and quantification of human herpesvirus 6 DNA in patients undergoing allogeneic haematopoietic stem cell transplantation.Memorias do Instituto Oswaldo Cruz · 2015Article
- Laboratory and clinical aspects of human herpesvirus 6 infections.Clinical microbiology reviews · 2015Review
- Effect of handling and storage conditions and stabilizing agent on the recovery of viral RNA from oral fluid of pigs.Journal of virological methods · 2014Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A real-time quantitative PCR assay has been developed to measure human herpesvirus 6 (HHV-6) DNA in biological specimens. The assay sensitivity was 10 copies of DNA per well, with a linear dynamic range of 10 to 10(7) copies of HHV-6 DNA. Intra- and interassay variations were, respectively, 0.88 and 0.8% for samples containing 10(2) DNA copies, 0.99 and 0.96% for samples containing 10(4) copies, and 0.76 and 0.9% for samples containing 10(6) copies. Among 34 saliva samples from healthy subjects, 26 were found to contain HHV-6 DNA (76.5%; median, 23,870 copies/ml), and following a single freeze-thaw cycle, 25 of the same samples were found to be positive for HHV-6 DNA, although at a statistically significantly lower concentration (median, 3,497 copies/ml). The assay enabled detection of HHV-6 DNA in lymph node biopsies from patients with Hodgkin's disease (HD) (13 of 37 patients [35.1%]), B-cell neoplasms (8 of 36 patients [22.2%]), and T- or NK-cell neoplasms (3 of 13 patients [23.1%]), with concentrations ranging from 100 to 864,640 HHV-6 copies per microg of DNA (HHV-6B being found in every case except two). All HD patients infected with HHV-6 presented clinically with the nodular sclerosis subtype of HD. The real-time quantitative PCR assay developed here was simple to perform and was sensitive over a wide range of HHV-6 concentrations. It therefore appears to be of potential value in clinical investigation or diagnosis of HHV-6 infection.
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