ArticleJournal of clinical microbiology2002
Characterization of Salmonella serovars by PCR-single-strand conformation polymorphism analysis.
Article in Journal of clinical microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Microbial diversity of landslide soils assessed by RFLP and SSCP fingerprints.Journal of applied genetics · 2014Article
- Aeromonas detection and characterization using genus-specific PCR and single-strand conformation polymorphism (SSCP).World journal of microbiology & biotechnology · 2012Article
- Differentiation of Erwinia amylovora and Erwinia pyrifoliae strains with single nucleotide polymorphisms and by synthesis of dihydrophenylalanine.Current microbiology · 2012Article
- One-step triplex high-resolution melting analysis for rapid identification and simultaneous subtyping of frequently isolated Salmonella serovars.Applied and environmental microbiology · 2012Article
- The analysis of groEL gene in Salmonella enterica subspecies enterica serovar Typhimurium isolated from avians by PCR-Restriction Fragment Length Polymorphism method.Veterinary research communications · 2011Article
- Comparison of classical serotyping and PremiTest assay for routine identification of common Salmonella enterica serovars.Journal of clinical microbiology · 2008Article
- Molecular typing of Salmonella enterica serovars Enteritidis, Corvallis, Anatum and Typhimurium from food and human stool samples in Tunisia, 2001-2004.Epidemiology and infection · 2008Article
- Multiplex PCR-based method for identification of common clinical serotypes of Salmonella enterica subsp. enterica.Journal of clinical microbiology · 2006Article
- Multiplex PCR for distinguishing the most common phase-1 flagellar antigens of Salmonella spp.Journal of clinical microbiology · 2004Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
PCR-restriction fragment length polymorphism (PCR-RFLP) and PCR-single-strand conformation polymorphism (PCR-SSCP) analyses were carried out on the 1.6-kb groEL gene from 41 strains of 10 different Salmonella serovars. Three HaeIII RFLP profiles were recognized, but no discrimination between the serovars could be achieved by this technique. However, PCR-SSCP analysis of the groEL genes of various Salmonella serovars produced 14 SSCP profiles, indicating the potential of this technique to differentiate different Salmonella serovars (interserovar differentiation). Moreover, PCR-SSCP could differentiate strains within a subset of serovars (intraserovar discrimination), as three SSCP profiles were produced for the 11 Salmonella enterica serovar Enteritidis strains, and two SSCP profiles were generated for the 7 S. enterica serovar Infantis and five S. enterica serovar Newport strains. PCR-SSCP has the potential to complement classical typing methods such as serotyping and phage typing for the typing of Salmonella serovars due to its rapidity, simplicity, and typeability.
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