ArticleJournal of clinical microbiology2002
Detection and differentiation of Cryptosporidium parasites that are pathogenic for humans by real-time PCR.
Article in Journal of clinical microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed.
- Comparison of Three Real-Time PCR Assays Targeting the SSU rRNA Gene, the COWP Gene and the DnaJ-Like Protein Gene for the Diagnosis ofPathogens (Basel, Switzerland) · 2021Article
- Species-level identification of trypanosomes infecting Australian wildlife by High-Resolution Melting - Real Time Quantitative Polymerase Chain Reaction (HRM-qPCR).International journal for parasitology. Parasites and wildlife · 2020Article
- Multicenter Comparative Study of SixMicroorganisms · 2020Article
- Enteric parasitic infection disturbs bacterial structure in Mexican children with autoantibodies for type 1 diabetes and/or celiac disease.Gut pathogens · 2020Article
- Ruling out nosocomial transmission of Cryptosporidium in a renal transplantation unit: case report.BMC infectious diseases · 2016Article
- Development and Evaluation of Three Real-Time PCR Assays for Genotyping and Source Tracking Cryptosporidium spp. in Water.Applied and environmental microbiology · 2015Article
- A novel ELISA test for laboratory diagnosis of Blastocystis spp. in human stool specimens.Parasitology research · 2015Article
- DNA extraction from protozoan oocysts/cysts in feces for diagnostic PCR.The Korean journal of parasitology · 2014Article
- Molecular testing for clinical diagnosis and epidemiological investigations of intestinal parasitic infections.Clinical microbiology reviews · 2014Review
- Molecular Characterization of Cryptosporidium spp. Isolated From Immunocompromised Patients and Children.Jundishapur journal of microbiology · 2014Article
- Real-time PCR assay targeting the actin gene for the detection of Cryptosporidium parvum in calf fecal samples.Parasitology research · 2012Article
- Detection and differentiation of Cryptosporidium spp. in human clinical samples by use of real-time PCR.Journal of clinical microbiology · 2011Article
- Diagnosis of parasitic diseases: old and new approaches.Interdisciplinary perspectives on infectious diseases · 2009Article
- Molecular characterization of Cryptosporidium isolates from human and bovine using 18s rRNA gene in Shahriar county of Tehran, Iran.Parasitology research · 2008Article
- Rapid microsphere assay for identification of cryptosporidium hominis and cryptosporidium parvum in stool and environmental samples.Journal of clinical microbiology · 2007Article
- Effectiveness of standard UV depuration at inactivating Cryptosporidium parvum recovered from spiked Pacific oysters (Crassostrea gigas).Applied and environmental microbiology · 2007Article
- Quantitative detection and differentiation of free-living amoeba species using SYBR green-based real-time PCR melting curve analysis.Current microbiology · 2006Article
- Rapid and sensitive detection of single cryptosporidium oocysts from archived glass slides.Journal of clinical microbiology · 2006Article
- Rapid, sensitive, and discriminating identification of Naegleria spp. by real-time PCR and melting-curve analysis.Applied and environmental microbiology · 2006Article
- Nucleic acid amplification-based techniques for pathogen detection and identification.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2006Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cryptosporidiosis is a significant cause of food-borne and waterborne outbreaks of diarrheal diseases. To better understand the route of transmission of Cryptosporidium parasites, a number of genotyping techniques have been developed, based on PCR-restriction fragment length polymorphism or sequencing analysis of antigen, structural, and housekeeping genes. In this study, a real-time assay for the detection of Cryptosporidium oocysts is described. This technique had a detection limit of five oocysts. By melting curve analysis of PCR products with fluorescence-labeled hybridization probes, this technique was able to differentiate five common Cryptosporidium parasites that are pathogenic for humans in a single PCR. We evaluated and validated the test using samples from presently known Cryptosporidium parasites that are pathogenic for humans. This technique provides an alternative molecular tool in epidemiologic studies of human cryptosporidiosis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.