ArticleJournal of clinical microbiology2002
Rapid detection and quantification of RNA of Ebola and Marburg viruses, Lassa virus, Crimean-Congo hemorrhagic fever virus, Rift Valley fever virus, dengue virus, and yellow fever virus by real-time reverse transcription-PCR.
Article in Journal of clinical microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 275 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
275 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Sensitivity and specificity of diagnostic tests for Lassa fever: a systematic review.BMC infectious diseases · 2019Pooled it
- Differential innate immune activation by wild-type and NSs-deficient RVFV strains in human blood monocytes.Emerging microbes & infections · 2026Article
- Lassa Virus-A Persistent and Evolving Threat.Microorganisms · 2026Review
- Evaluation of the Collection Efficiency of a Wet-Type Electrostatic Precipitator for Aerosolized Influenza A Virus.Microorganisms · 2026Article
- Species composition, abundance and seasonality of potential Rift Valley fever virus vectors in north-eastern KwaZulu-Natal province, South Africa.Parasites & vectors · 2026Article
- Crimean-Congo hemorrhagic fever virus in the last four decades (1981-2025): global output, collaboration networks, and emerging frontiers.Tropical animal health and production · 2026Article
- Intranasal infection of Rift Valley fever virus in the ferret model suggests multiple routes of neuroinvasion with consequential encephalitis and ophthalmitis.Scientific reports · 2026Article
- Technical validation and implementation of a multiplex real-time PCR for differential diagnostic detection of Zika virus, yellow fever virus, dengue virus and chikungunya virus (ZYDC-PCR).BMC infectious diseases · 2026Article
- Cross-border introduction risk of Rift Valley fever virus: surveillance evidence from intercepted livestock in southwestern Saudi Arabia.Frontiers in veterinary science · 2026Article
- Entomological investigations into yellow fever outbreak in northern Ghana.PLOS global public health · 2026Article
- Rift Valley fever seroprevalence in ruminants in Dhobley town, Lower Juba region, Somalia, in 2021.Epidemiology and infection · 2025Article
- Technical validation of a multiplex real-time PCR for combined detection of Rift Valley fever, chikungunya, Zika and dengue viruses.Journal of virological methods · 2025Article
- Investigating Oropouche as a Possible Etiology for Febrile Illness in a Clinical Cohort from Colombia, 2014-2015.The American journal of tropical medicine and hygiene · 2025Article
- Detection of Borrelia burgdorferi (s.l.) in Ixodes ricinus ticks collected in Iceland.Parasites & vectors · 2025Article
- Article
- Dengue and chikungunya viruses among febrile travelers entering Iran (2015-2021): Evidence of multiple introductions from different countries.IJID regions · 2025Article
- Excellent capability for molecular detection ofJournal of clinical microbiology · 2025Article
- Molecular Diagnosis of Phlebovirus riftense Infection Using an L-Segment-Based Real-Time RT-PCR Method.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Vector Competence Assays for RVFV in Mosquitoes.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Serological Evidence of Cryptic Rift Valley Fever Virus Transmission Among Humans and Livestock in Central Highlands of Kenya.Viruses · 2024Article
215 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Viral hemorrhagic fevers (VHFs) are acute infections with high case fatality rates. Important VHF agents are Ebola and Marburg viruses (MBGV/EBOV), Lassa virus (LASV), Crimean-Congo hemorrhagic fever virus (CCHFV), Rift Valley fever virus (RVFV), dengue virus (DENV), and yellow fever virus (YFV). VHFs are clinically difficult to diagnose and to distinguish; a rapid and reliable laboratory diagnosis is required in suspected cases. We have established six one-step, real-time reverse transcription-PCR assays for these pathogens based on the Superscript reverse transcriptase-Platinum Taq polymerase enzyme mixture. Novel primers and/or 5'-nuclease detection probes were designed for RVFV, DENV, YFV, and CCHFV by using the latest DNA database entries. PCR products were detected in real time on a LightCycler instrument by using 5'-nuclease technology (RVFV, DENV, and YFV) or SybrGreen dye intercalation (MBGV/EBOV, LASV, and CCHFV). The inhibitory effect of SybrGreen on reverse transcription was overcome by initial immobilization of the dye in the reaction capillaries. Universal cycling conditions for SybrGreen and 5'-nuclease probe detection were established. Thus, up to three assays could be performed in parallel, facilitating rapid testing for several pathogens. All assays were thoroughly optimized and validated in terms of analytical sensitivity by using in vitro-transcribed RNA. The >or=95% detection limits as determined by probit regression analysis ranged from 1,545 to 2,835 viral genome equivalents/ml of serum (8.6 to 16 RNA copies per assay). The suitability of the assays was exemplified by detection and quantification of viral RNA in serum samples of VHF patients.
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