ArticleApplied and environmental microbiology2002
Molecular characterization of the S-layer gene, sbpA, of Bacillus sphaericus CCM 2177 and production of a functional S-layer fusion protein with the ability to recrystallize in a defined orientation while presenting the fused allergen.
Article in Applied and environmental microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 126 citations in OpenAlex.
- AnaToc75 (Alr2269) mediates calcium uptake across the outer membrane inJournal of bacteriology · 2025Article
- A new age in structural S-layer biology: Experimental and in silico milestones.The Journal of biological chemistry · 2025Review
- Scalable biomimetic sensing system with membrane receptor dual-monolayer probe and graphene transistor arrays.Science advances · 2023Article
- A New Method for Dispersing Pristine Carbon Nanotubes Using Regularly Arranged S-Layer Proteins.Nanomaterials (Basel, Switzerland) · 2021Article
- Effect of Spheroidal Age on Sorafenib Diffusivity and Toxicity in a 3D HepG2 Spheroid Model.Scientific reports · 2019Article
- In-situ 2D bacterial crystal growth as a function of protein concentration: An atomic force microscopy study.Microscopy research and technique · 2018Article
- Microbial Delivery Vehicles for Allergens and Allergen-Derived Peptides in Immunotherapy of Allergic Diseases.Frontiers in microbiology · 2018Review
- Lactobacillus buchneri S-layer as carrier for an Ara h 2-derived peptide for peanut allergen-specific immunotherapy.Molecular immunology · 2017Article
- In Vitro Characterization of the Two-Stage Non-Classical Reassembly Pathway of S-Layers.International journal of molecular sciences · 2017Article
- Symmetry based assembly of a 2 dimensional protein lattice.PloS one · 2017Article
- S-layer fusion protein as a tool functionalizing emulsomes and CurcuEmulsomes for antibody binding and targeting.Colloids and surfaces. B, Biointerfaces · 2015Article
- Ion-specific control of the self-assembly dynamics of a nanostructured protein lattice.ACS nano · 2015Article
- S-layers: principles and applications.FEMS microbiology reviews · 2014Article
- S-layer protein self-assembly.International journal of molecular sciences · 2013Article
- Increased imaging speed and force sensitivity for bio-applications with small cantilevers using a conventional AFM setup.Micron (Oxford, England : 1993) · 2012Article
- Relevant uses of surface proteins--display on self-organized biological structures.Microbial biotechnology · 2012Review
- Production, secretion, and cell surface display of recombinant Sporosarcina ureae S-layer fusion proteins in Bacillus megaterium.Applied and environmental microbiology · 2012Article
- S-layer fusion proteins--construction principles and applications.Current opinion in biotechnology · 2011Review
- Fast and easy protocol for the purification of recombinant S-layer protein for synthetic biology applications.Biotechnology journal · 2011Article
- Expression of an endotoxin-free S-layer/allergen fusion protein in gram-positive Bacillus subtilis 1012 for the potential application as vaccines for immunotherapy of atopic allergy.Microbial cell factories · 2011Article
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Authors and funding
6 authors at 1 institution.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The nucleotide sequence encoding the crystalline bacterial cell surface (S-layer) protein SbpA of Bacillus sphaericus CCM 2177 was determined by a PCR-based technique using four overlapping fragments. The entire sbpA sequence indicated one open reading frame of 3,804 bp encoding a protein of 1,268 amino acids with a theoretical molecular mass of 132,062 Da and a calculated isoelectric point of 4.69. The N-terminal part of SbpA, which is involved in anchoring the S-layer subunits via a distinct type of secondary cell wall polymer to the rigid cell wall layer, comprises three S-layer-homologous motifs. For screening of amino acid positions located on the outer surface of the square S-layer lattice, the sequence encoding Strep-tag I, showing affinity to streptavidin, was linked to the 5' end of the sequence encoding the recombinant S-layer protein (rSbpA) or a C-terminally truncated form (rSbpA(31-1068)). The deletion of 200 C-terminal amino acids did not interfere with the self-assembly properties of the S-layer protein but significantly increased the accessibility of Strep-tag I. Thus, the sequence encoding the major birch pollen allergen (Bet v1) was fused via a short linker to the sequence encoding the C-terminally truncated form rSpbA(31-1068). Labeling of the square S-layer lattice formed by recrystallization of rSbpA(31-1068)/Bet v1 on peptidoglycan-containing sacculi with a Bet v1-specific monoclonal mouse antibody demonstrated the functionality of the fused protein sequence and its location on the outer surface of the S-layer lattice. The specific interactions between the N-terminal part of SbpA and the secondary cell wall polymer will be exploited for an oriented binding of the S-layer fusion protein on solid supports to generate regularly structured functional protein lattices.
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