ArticleRNA (New York, N.Y.)2002
Modification of the universally unmodified uridine-33 in a mitochondria-imported edited tRNA and the role of the anticodon arm structure on editing efficiency.
Article in RNA (New York, N.Y.), 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 50 citations in OpenAlex.
- The mitochondrial proteome of diplonemids: from conventional pathways to eccentric RNA editing and transcript processing.BMC genomics · 2025Article
- Cryo-EM structures of the human Elongator complex at work.Nature communications · 2024Article
- Article
- Comparative patterns of modified nucleotides in individual tRNA species from a mesophilic and two thermophilic archaea.RNA (New York, N.Y.) · 2020Article
- From canonical to modified nucleotides: balancing translation and metabolism.Critical reviews in biochemistry and molecular biology · 2020Review
- Naturally occurring modified ribonucleosides.Wiley interdisciplinary reviews. RNA · 2020Review
- The single CCA-adding enzyme ofThe Journal of biological chemistry · 2020Article
- How the intracellular partitioning of tRNA and tRNA modification enzymes affects mitochondrial function.IUBMB life · 2018Review
- The role of intracellular compartmentalization on tRNA processing and modification.RNA biology · 2018Review
- Recent Advances in Our Understanding of the Biosynthesis of Sulfur Modifications in tRNAs.Frontiers in microbiology · 2018Article
- Binding synergy as an essential step for tRNA editing and modification enzyme codependence inRNA (New York, N.Y.) · 2018Article
- Biosynthesis of Sulfur-Containing tRNA Modifications: A Comparison of Bacterial, Archaeal, and Eukaryotic Pathways.Biomolecules · 2017Review
- Article
- tRNA biology in mitochondria.International journal of molecular sciences · 2015Review
- Unusual noncanonical intron editing is important for tRNA splicing in Trypanosoma brucei.Molecular cell · 2013Article
- Determinants of tRNA editing and modification: avoiding conundrums, affecting function.Seminars in cell & developmental biology · 2012Review
- Assembling Fe/S-clusters and modifying tRNAs: ancient co-factors meet ancient adaptors.Trends in parasitology · 2011Article
- Crystal structures of three protozoan homologs of tryptophanyl-tRNA synthetase.Molecular and biochemical parasitology · 2011Article
- The Fe/S cluster assembly protein Isd11 is essential for tRNA thiolation in Trypanosoma brucei.The Journal of biological chemistry · 2010Article
- Thiolated tRNAs of Trypanosoma brucei are imported into mitochondria and dethiolated after import.The Journal of biological chemistry · 2009Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Editing of tRNA has a wide phylogenetic distribution among eukaryotes and in some cases serves to expand the decoding capacity of the target tRNA. We previously described C-to-U editing of the wobble position of the imported tRNA(Trp) in Leishmania mitochondria, which is essential for decoding UGA codons as tryptophan. Here we show the complete set of nucleotide modifications in the anticodon arm of the mitochondrial and cytosolic tRNA(Trp) as determined by electrospray ionization mass spectrometry. This analysis revealed extensive mitochondria-specific posttranscriptional modifications, including the first example of thiolation of U33, the "universally unmodified" uridine. In light of the known rigidity imparted on sugar conformation by thiolation, our discovery of a thiolated U33 suggests that conformational flexibility is not a universal feature of the anticodon structural signature. In addition, the in vivo analysis of tRNA(Trp) variants presented shows a single base-pair reversal in the anticodon stem of tRNA(Trp) is sufficient to abrogate editing in vivo, indicating that subtle changes in anticodon structure can have drastic effects on editing efficiency.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.