ArticleNucleic acids research2002
Structural perturbations in DNA caused by bis-intercalation of ditercalinium visualised by atomic force microscopy.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 72 citations in OpenAlex.
- Tools shaping drug discovery and development.Biophysics reviews · 2022Review
- Modulating the chemo-mechanical response of structured DNA assemblies through binding molecules.Nucleic acids research · 2021Article
- Measuring the wall depletion length of nanoconfined DNA.The Journal of chemical physics · 2018Article
- Article
- Characterizing the interaction between DNA and GelRed fluorescent stain.European biophysics journal : EBJ · 2015Article
- PT-ACRAMTU, a platinum-acridine anticancer agent, lengthens and aggregates, but does not stiffen or soften DNA.Cell biochemistry and biophysics · 2013Article
- Exploring the interaction of ruthenium(II) polypyridyl complexes with DNA using single-molecule techniques.Langmuir : the ACS journal of surfaces and colloids · 2006Article
- Studying the effect of a charged surface on the interaction of bleomycin with DNA using an atomic force microscope.European biophysics journal : EBJ · 2005Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Atomic force microscopy (AFM) has been used to examine perturbations in the tertiary structure of DNA induced by the binding of ditercalinium, a DNA bis-intercalator with strong anti-tumour properties. We report AFM images of plasmid DNA of both circular and linearised forms showing a difference in the formation of supercoils and plectonemic coils caused at least in part by alterations in the superhelical stress upon bis-intercalation. A further investigation of the effects of drug binding performed with 292 bp mixed-sequence DNA fragments, and using increment in contour length as a reliable measure of intercalation, revealed saturation occurring at a point where sufficient drug was present to interact with every other available binding site. Moment analysis based on the distribution of angles between segments along single DNA molecules showed that at this level of bis-intercalation, the apparent persistence length of the molecules was 91.7 +/- 5.7 nm, approximately twice as long as that of naked DNA. We conclude that images of single molecules generated using AFM provide a valuable supplement to solution-based techniques for evaluation of physical properties of biological macromolecules.
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