ArticleNucleic acids research2002
Pentamidine inhibits catalytic activity of group I intron Ca.LSU by altering RNA folding.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 35 citations in OpenAlex.
- Group I introns: Structure, splicing and their applications in medical mycology.Genetics and molecular biology · 2024Article
- Distribution and Polymorphisms of Group I Introns in Mitochondrial Genes fromJournal of fungi (Basel, Switzerland) · 2023Article
- Understanding the characteristics of nonspecific binding of drug-like compounds to canonical stem-loop RNAs and their implications for functional cellular assays.RNA (New York, N.Y.) · 2021Article
- Regulation of MALAT1 triple helix stability and in vitro degradation by diphenylfurans.Nucleic acids research · 2020Article
- Mitigating RNA Toxicity in Myotonic Dystrophy using Small Molecules.International journal of molecular sciences · 2019Review
- Polymorphism in Mitochondrial Group I Introns amongFrontiers in microbiology · 2018Article
- Small Molecules Which Improve Pathogenesis of Myotonic Dystrophy Type 1.Frontiers in neurology · 2018Review
- Identifying the preferred RNA motifs and chemotypes that interact by probing millions of combinations.Nature communications · 2012Article
- Evaluating target silencing by short hairpin RNA mediated by the group I intron in cultured mammalian cells.BMC biotechnology · 2011Article
- Pentamidine inhibits Coxiella burnetii growth and 23S rRNA intron splicing in vitro.International journal of antimicrobial agents · 2010Article
- Pentamidine reverses the splicing defects associated with myotonic dystrophy.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Slow formation of a pseudoknot structure is rate limiting in the productive co-transcriptional folding of the self-splicing Candida intron.RNA (New York, N.Y.) · 2009Article
- Pentamidine binds to tRNA through non-specific hydrophobic interactions and inhibits aminoacylation and translation.Nucleic acids research · 2008Article
- In vitro selection and in vivo efficacy of piperazine- and alkanediamide-linked bisbenzamidines against Pneumocystis pneumonia in mice.Antimicrobial agents and chemotherapy · 2006Article
- Highly active anti-Pneumocystis carinii compounds in a library of novel piperazine-linked bisbenzamidines and related compounds.Antimicrobial agents and chemotherapy · 2004Article
- Concerted folding of a Candida ribozyme into the catalytically active structure posterior to a rapid RNA compaction.Nucleic acids research · 2003Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The antimicrobial agent pentamidine inhibits the self-splicing of the group I intron Ca.LSU from the transcripts of the 26S rRNA gene of Candida albicans, but the mechanism of pentamidine inhibition is not clear. We show that preincubation of the ribozyme with pentamidine enhances the inhibitory effect of the drug and alters the folding of the ribozyme in a pattern varying with drug concentration. Pentamidine at 25 microM prevents formation of the catalytically active F band conformation of the precursor RNA and alters the ribonuclease T1 cleavage pattern of Ca.LSU RNA. The effects on cleavage suggest that pentamidine mainly binds to specific sites in or near asymmetric loops of helices P2 and P2.1 on the ribozyme, as well as to the tetraloop of P9.2 and the loosely paired helix P9, resulting in an altered structure of helix P7, which contains the active site. Positively charged molecules antagonize pentamidine inhibition of catalysis and relieve the drug effect on ribozyme folding, suggesting that pentamidine binds to a magnesium binding site(s) of the ribozyme to exert its inhibitory effect.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.