ArticleNucleic acids research2002
Analysis of cell-cycle gene expression in Saccharomyces cerevisiae using microarrays and multiple synchronization methods.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed.
- PAGE: an R package for network detection of multivariate error-prone gene expression data with the availability of auxiliary information.BMC bioinformatics · 2026Article
- Cryptococcus neoformans Slu7 ensures nuclear positioning during mitotic progression through RNA splicing.PLoS genetics · 2024Article
- Using extremal events to characterize noisy time series.Journal of mathematical biology · 2020Article
- Translational control of lipogenic enzymes in the cell cycle of synchronous, growing yeast cells.The EMBO journal · 2017Article
- Cell cycle population effects in perturbation studies.Molecular systems biology · 2014Article
- Identification of under-detected periodicity in time-series microarray data by using empirical mode decomposition.PloS one · 2014Article
- How to cluster gene expression dynamics in response to environmental signals.Briefings in bioinformatics · 2012Article
- Article
- Principal-oscillation-pattern analysis of gene expression.PloS one · 2012Article
- High-throughput tracking of single yeast cells in a microfluidic imaging matrix.Lab on a chip · 2011Article
- Gene expression model (in)validation by Fourier analysis.BMC systems biology · 2010Article
- Model-based deconvolution of cell cycle time-series data reveals gene expression details at high resolution.PLoS computational biology · 2009Article
- Optimization of yeast cell cycle analysis and morphological characterization by multispectral imaging flow cytometry.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2008Article
- High-resolution timing of cell cycle-regulated gene expression.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Revealing cell cycle control by combining model-based detection of periodic expression with novel cis-regulatory descriptors.BMC systems biology · 2007Article
- Bottleneck genes and community structure in the cell cycle network of S. pombe.PLoS computational biology · 2007Article
- Article
- Bayesian detection of periodic mRNA time profiles without use of training examples.BMC bioinformatics · 2006Article
- Identification of significant periodic genes in microarray gene expression data.BMC bioinformatics · 2005Article
- Microarray analysis of gene expression during the cell cycle.Cell & chromosome · 2003Article
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Abstract
Microarray analysis of gene expression during the yeast division cycle has led to the proposal that a significant number of genes in Saccharomyces cerevisiae are expressed in a cell-cycle-specific manner. Four different methods of synchronization were used for cell-cycle analysis. Randomized data exhibit periodic patterns of lesser strength than the experimental data. Thus the cyclicities in the expression measurements in the four experiments presented do not arise from chance fluctuations or noise in the data. However, when the degree of cyclicity for genes in different experiments are compared, a large degree of non-reproducibility is found. Re-examining the phase timing of peak expression, we find that three of the experiments (those using alpha-factor, CDC28 and CDC15 synchronization) show consistent patterns of phasing, but the elutriation synchrony results demonstrate a different pattern from the other arrest-release synchronization methods. Specific genes can show a wide range of cyclical behavior between different experiments; a gene with high cyclicity in one experiment can show essentially no cyclicity in another experiment. The elutriation experiment, possibly being the least perturbing of the four synchronization methods, may give the most accurate characterization of the state of gene expression during the normal, unperturbed cell cycle. Under this alternative explanation, the observed cyclicities in the other three experiments are a stress response to synchronization, and may not reproduce in unperturbed cells.
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