ArticleNucleic acids research2002
The mitochondrial DNA polymerase as a target of oxidative damage.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
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Who cites it
70 citing papers in PubMed, 185 citations in OpenAlex.
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- Effect of Denervation on Skeletal Muscle Mitochondria in Heterozygous mtDNA Mutator Mice.FASEB bioAdvances · 2026Article
- Oxidative Damage Induced Disruption of Mitochondrial Quality Control Underlying Abnormal Energy Metabolism in Peripheral Leukocytes of Schizophrenia Patients.Schizophrenia bulletin open · 2026Article
- Mitochondria in oxidative stress, inflammation and aging: from mechanisms to therapeutic advances.Signal transduction and targeted therapy · 2025Review
- Ergothioneine Improves the Quality of Boar Sperm DuringAnimals : an open access journal from MDPI · 2025Article
- Comparative effects of 3,5-diiodo-L-thyronine and 3,5,3'-triiodo-L-thyronine on mitochondrial damage and cGAS/STING-driven inflammation in liver of hypothyroid rats.Frontiers in endocrinology · 2024Article
- The role of TEMRA cell-mediated immune senescence in the development and treatment of HIV disease.Frontiers in immunology · 2023Review
- Vitamin K2 Modulates Mitochondrial Dysfunction Induced by 6-Hydroxydopamine in SH-SY5Y Cells via Mitochondrial Quality-Control Loop.Nutrients · 2022Article
- Oxidative Stress and Energy Metabolism in the Brain: Midlife as a Turning Point.Antioxidants (Basel, Switzerland) · 2021Review
- Mitochondrial DNA-depleter mouse as a model to study human pigmentary skin disorders.Pigment cell & melanoma research · 2021Article
- The Impact of HIV- and ART-Induced Mitochondrial Dysfunction in Cellular Senescence and Aging.Cells · 2021Review
- The Uprising of Mitochondrial DNA Biomarker in Cancer.Disease markers · 2021Review
- Protective effects ofToxicology reports · 2021Article
- Role of Commensal Microbes in the γ-Ray Irradiation-Induced Physiological Changes inMicroorganisms · 2020Article
- Placental function in maternal obesity.Clinical science (London, England : 1979) · 2020Review
- Oxidative damage diminishes mitochondrial DNA polymerase replication fidelity.Nucleic acids research · 2020Article
- Combined Effects of MMP-7, MMP-8 and MMP-26 on the Risk of Ischemic Stroke.Journal of clinical medicine · 2019Article
- Base-excision repair deficiency alone or combined with increased oxidative stress does not increase mtDNA point mutations in mice.Nucleic acids research · 2018Article
- Mechanisms and Modulation of Oxidative/Nitrative Stress in Type 4 Cardio-Renal Syndrome and Renal Sarcopenia.Frontiers in physiology · 2018Review
- Significant Association Between Low Mitochondrial DNA Content in Peripheral Blood Leukocytes and Ischemic Stroke.Journal of the American Heart Association · 2017Article
10 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The mitochondrial respiratory chain is a source of reactive oxygen species (ROS) that are responsible for oxidative modification of biomolecules, including proteins. Due to its association with mitochondrial DNA, DNA polymerase gamma (pol gamma) is in an environment to be oxidized by hydrogen peroxide and hydroxyl radicals that may be generated in the presence of iron ions associated with DNA. We tested whether human pol gamma was a possible target of ROS with H2O2 and investigated the effect on the polymerase activities and DNA binding efficiency. A 1 h treatment with 250 microM H2O2 significantly inhibited DNA polymerase activity of the p140 subunit and lowered its DNA binding efficiency. Addition of p55 to the p140 catalytic subunit prior to H2O2 treatment offered protection from oxidative inactivation. Oxidatively modified amino acid residues in pol gamma resulting from H2O2 treatment were observed in vitro as well as in vivo, in SV40-transfected human fibroblasts. Pol gamma was detected as one of the major oxidized mitochondrial matrix proteins, with a detectable decline in polymerase activity. These results suggest pol gamma as a target of oxidative damage, which may result in a reduction in mitochondrial DNA replication and repair capacities.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.