ArticleThe Biochemical journal2002
Identification of a novel AU-rich-element-binding protein which is related to AUF1.
Article in The Biochemical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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Who cites it
22 citing papers in PubMed, 55 citations in OpenAlex.
- Cellular hnRNPAB binding to viral nucleoprotein inhibits flu virus replication by blocking nuclear export of viral mRNA.iScience · 2021Article
- Dynamic mRNP Remodeling in Response to Internal and External Stimuli.Biomolecules · 2020Review
- Sorting mRNA Molecules for Cytoplasmic Transport and Localization.Frontiers in genetics · 2018Review
- Clinical Significance and Biological Role of HuR in Head and Neck Carcinomas.Disease markers · 2018Review
- Suppression of HPV-16 late L1 5'-splice site SD3632 by binding of hnRNP D proteins and hnRNP A2/B1 to upstream AUAGUA RNA motifs.Nucleic acids research · 2013Article
- Article
- The transacting factor CBF-A/Hnrnpab binds to the A2RE/RTS element of protamine 2 mRNA and contributes to its translational regulation during mouse spermatogenesis.PLoS genetics · 2013Article
- Article
- Hnrpab regulates neural development and neuron cell survival after glutamate stimulation.RNA (New York, N.Y.) · 2012Article
- In neurons, activity-dependent association of dendritically transported mRNA transcripts with the transacting factor CBF-A is mediated by A2RE/RTS elements.Molecular biology of the cell · 2011Article
- Characterization of nuclear factors modulating the apolipoprotein D promoter during growth arrest: implication of PARP-1, APEX-1 and ERK1/2 catalytic activities.Biochimica et biophysica acta · 2010Article
- Posttranscriptional Regulation of Cyclooxygenase 2 Expression in Colorectal Cancer.Current colorectal cancer reports · 2010Article
- Regulation of transforming growth factor-beta-dependent cyclooxygenase-2 expression in fibroblasts.The Journal of biological chemistry · 2009Article
- Three proteins of the U7-specific Sm ring function as the molecular ruler to determine the site of 3'-end processing in mammalian histone pre-mRNA.Molecular and cellular biology · 2009Article
- In cultured oligodendrocytes the A/B-type hnRNP CBF-A accompanies MBP mRNA bound to mRNA trafficking sequences.Molecular biology of the cell · 2008Article
- NRF IRES activity is mediated by RNA binding protein JKTBP1 and a 14-nt RNA element.RNA (New York, N.Y.) · 2007Article
- A proximal activator of transcription in epithelial-mesenchymal transition.The Journal of clinical investigation · 2007Article
- 40LoVe interacts with Vg1RBP/Vera and hnRNP I in binding the Vg1-localization element.RNA (New York, N.Y.) · 2006Article
- Alternative polyadenylation of cyclooxygenase-2.Nucleic acids research · 2005Article
- Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The AU-rich element (ARE) is an important instability determinant for a large number of early-response-gene mRNAs. AREs also mediate the stabilization of certain pro-inflammatory mRNAs, such as tumour necrosis factor (TNF)-alpha and cyclo-oxygenase-2 (COX-2), in response to inflammatory stimuli. To understand how AREs control mRNA stability, it is necessary to identify trans-acting factors. We have purified a new ARE-binding protein and identified it as CArG box-binding factor-A (CBF-A). The amino acid sequence of CBF-A is highly similar to that of the ARE-binding protein AUF1. Recombinant CBF-A bound the COX-2 and TNF-alpha AREs, but not a non-specific control RNA. In contrast, in an electrophoretic-mobility-shift assay (EMSA) of crude RAW 264.7 macrophage-like cell extracts, an antiserum that recognizes both AUF1 and CBF-A failed to supershift complexes formed on the TNF-alpha ARE, but did supershift a complex specific for the COX-2 ARE. CBF-A exists as two isoforms, p37 and p42, that differ by a 47-amino-acid insertion close to the C-terminus. By expressing epitope-tagged isoforms of CBF-A it was shown that the p42 isoform binds the COX-2 ARE in EMSA of crude cell extracts. In a HeLa-cell tetracycline-regulated reporter system, overexpression of the p42 CBF-A isoform resulted in stabilization of a COX-2 ARE reporter mRNA. Epitope-tagged p42 CBF-A expressed in HeLa cells co-immunoprecipitated with endogenous COX-2 mRNA, but not glyceraldehyde-3-phosphate dehydrogenase mRNA, as shown by reverse-transcription PCR. The similarity between CBF-A and AUF1 suggests that CBF-A could be re-named AUF2.
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Registered trials
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