ArticleGene therapy2002
Gene transduction efficiency in cells of different species by HIV and EIAV vectors.
Article in Gene therapy, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 81 citations in OpenAlex.
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- The NAD-dependent deacetylase sirtuin 2 is a suppressor of microglial activation and brain inflammation.The EMBO journal · 2013Article
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- Conformational adaptation of Asian macaque TRIMCyp directs lineage specific antiviral activity.PLoS pathogens · 2010Article
- Induced pluripotent reprogramming from promiscuous human stemness related factors.Clinical and translational science · 2009Article
- Rhesus macaque TRIM5 alleles have divergent antiretroviral specificities.Journal of virology · 2008Article
- Identification of an arsenic-sensitive block to primate lentiviral infection of human dendritic cells.Journal of virology · 2007Article
- An active TRIM5 protein in rabbits indicates a common antiviral ancestor for mammalian TRIM5 proteins.Journal of virology · 2007Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ability of human immunodeficiency virus (HIV)- and equine infectious anaemia virus (EIAV)-based vectors to transduce cell lines from a range of species was compared. Both vectors carried the vesicular stomatitis virus G (VSV-G) envelope protein and encoded an enhanced green fluorescent protein (eGFP) gene driven by a human cytomegalovirus (CMV) early promoter. Immunostaining for viral core proteins and VSV-G was used to demonstrate that the HIV and EIAV vector preparations contained similar numbers of virus particles. Various cell lines were transduced with these vectors and the transduction efficiency was estimated by measuring eGFP expression. Efficient transduction by both vectors was observed in human, hamster, pig, horse, cat and dog cell lines, although EIAV vector was about 10-fold less efficient in human, hamster and pig cells normalised to the total number of viral particles. This could be partly explained by the lower RNA genome levels per particle for EIAV as measured by real-time RT-PCR. Rodent cells appeared to be transduced inefficiently with both vectors, but when the CMV promoter was substituted with the EF1alpha promoter in the HIV vectors, the expression level increased leading to an increase in the measurable level of transduction.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.