ArticleProceedings of the National Academy of Sciences of the United States of America2002
Gene activation by interaction of an inhibitor with a cytoplasmic signaling protein.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 117 citations in OpenAlex.
- The galactokinase enzyme of yeast senses metabolic flux to stabilize galactose pathway regulation.Nature metabolism · 2025Article
- A unique metabolic gene cluster regulates lactose and galactose metabolism in the yeastApplied and environmental microbiology · 2024Article
- Review
- Sugar Sensing and Signaling inFrontiers in microbiology · 2019Review
- Assigning function to natural allelic variation via dynamic modeling of gene network induction.Molecular systems biology · 2018Article
- A Precise Genome Editing Method Reveals Insights into the Activity of Eukaryotic Promoters.Cell reports · 2017Article
- The 9aaTAD Is Exclusive Activation Domain in Gal4.PloS one · 2017Article
- Alcohol dehydrogenase gene ADH3 activates glucose alcoholic fermentation in genetically engineered Dekkera bruxellensis yeast.Applied microbiology and biotechnology · 2016Article
- Timing and Variability of Galactose Metabolic Gene Activation Depend on the Rate of Environmental Change.PLoS computational biology · 2015Article
- Nuclear localization of Haa1, which is linked to its phosphorylation status, mediates lactic acid tolerance in Saccharomyces cerevisiae.Applied and environmental microbiology · 2014Article
- Self-association of the Gal4 inhibitor protein Gal80 is impaired by Gal3: evidence for a new mechanism in the GAL gene switch.Molecular and cellular biology · 2013Article
- Regulations of sugar transporters: insights from yeast.Current genetics · 2013Review
- Molecular simulation and docking studies of Gal1p and Gal3p proteins in the presence and absence of ligands ATP and galactose: implication for transcriptional activation of GAL genes.Journal of computer-aided molecular design · 2012Article
- Interplay of a ligand sensor and an enzyme in controlling expression of the Saccharomyces cerevisiae GAL genes.Eukaryotic cell · 2012Article
- The Gal3p transducer of the GAL regulon interacts with the Gal80p repressor in its ligand-induced closed conformation.Genes & development · 2012Article
- Review
- Rapid GAL gene switch of Saccharomyces cerevisiae depends on nuclear Gal3, not nucleocytoplasmic trafficking of Gal3 and Gal80.Genetics · 2011Article
- Phosphoinositide [PI(3,5)P2] lipid-dependent regulation of the general transcriptional regulator Tup1.Genes & development · 2011Article
- Application of structure equation modeling for inferring a serial transcriptional regulation in yeast.Gene regulation and systems biology · 2011Article
- A general mechanism for network-dosage compensation in gene circuits.Science (New York, N.Y.) · 2010Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Galactose-inducible genes (GAL genes) in yeast Saccharomyces cerevisiae are efficiently transcribed only when the sequence-specific transcription activator Gal4p is activated. Activation of Gal4p requires the interaction between the Gal4p inhibitory protein Gal80p and the galactokinase paralog, Gal3p. It has been proposed that Gal3p binds to a Gal80p-Gal4p complex in the nucleus to activate Gal4p. Here, we present evidence that the Gal3p-Gal80p interaction occurs in the cytoplasm, and concurrently, Gal80p is removed from Gal4p at the GAL gene promoter. We also show that GAL gene expression can be activated by heterologous protein-protein interaction in the cytoplasm that is independent of galactose and Gal3p function. These results indicate that galactose-triggered Gal3p-Gal80p association in the cytoplasm activates Gal4p in the nucleus.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.