ArticleThe Journal of general physiology2002
Spontaneous transient outward currents arise from microdomains where BK channels are exposed to a mean Ca(2+) concentration on the order of 10 microM during a Ca(2+) spark.
Article in The Journal of general physiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed.
- Article
- Evaluation and experimental monitoring of calcium in plasma membrane inner nanoscale cytoplasm regions.Communications biology · 2025Article
- Article
- A computational model predicts sex-specific responses to calcium channel blockers in mammalian mesenteric vascular smooth muscle.bioRxiv : the preprint server for biology · 2024Article
- Effects of hypoxia on uteroplacental and fetoplacental vascular function during pregnancy.Frontiers in physiology · 2024Review
- CaInternational journal of molecular sciences · 2023Review
- STIM1-dependent peripheral coupling governs the contractility of vascular smooth muscle cells.eLife · 2022Article
- A junctophilin-caveolin interaction enables efficient coupling between ryanodine receptors and BKThe Journal of biological chemistry · 2019Article
- Regulation of BK Channels by Beta and Gamma Subunits.Annual review of physiology · 2019Review
- Differential contribution of CaThe Journal of general physiology · 2018Article
- Microtubule structures underlying the sarcoplasmic reticulum support peripheral coupling sites to regulate smooth muscle contractility.Science signaling · 2017Article
- Membrane depolarization activates BK channels through ROCK-mediated β1 subunit surface trafficking to limit vasoconstriction.Science signaling · 2017Article
- Regulation of cellular communication by signaling microdomains in the blood vessel wall.Pharmacological reviews · 2014Review
- The regulation of BK channel activity by pre- and post-translational modifications.Frontiers in physiology · 2014Review
- Ethanol-mediated relaxation of guinea pig urinary bladder smooth muscle: involvement of BK and L-type Ca2+ channels.American journal of physiology. Cell physiology · 2014Article
- Transduction of voltage and Ca2+ signals by Slo1 BK channels.Physiology (Bethesda, Md.) · 2013Review
- Examining the role of mitochondria in Ca²⁺ signaling in native vascular smooth muscle.Microcirculation (New York, N.Y. : 1994) · 2013Review
- The BK channel: a vital link between cellular calcium and electrical signaling.Protein & cell · 2012Review
- Regulation of gastrointestinal motility--insights from smooth muscle biology.Nature reviews. Gastroenterology & hepatology · 2012Review
- Controlling Ca2+-activated K+ channels with models of Ca2+ buffering in Purkinje cells.Cerebellum (London, England) · 2012Article
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4 authors.
Funding
Abstract
Ca(2+) sparks are small, localized cytosolic Ca(2+) transients due to Ca(2+) release from sarcoplasmic reticulum through ryanodine receptors. In smooth muscle, Ca(2+) sparks activate large conductance Ca(2+)-activated K(+) channels (BK channels) in the spark microdomain, thus generating spontaneous transient outward currents (STOCs). The purpose of the present study is to determine experimentally the level of Ca(2+) to which the BK channels are exposed during a spark. Using tight seal, whole-cell recording, we have analyzed the voltage-dependence of the STOC conductance (g((STOC))), and compared it to the voltage-dependence of BK channel activation in excised patches in the presence of different [Ca(2+)]s. The Ca(2+) sparks did not change in amplitude over the range of potentials of interest. In contrast, the magnitude of g((STOC)) remained roughly constant from 20 to -40 mV and then declined steeply at more negative potentials. From this and the voltage dependence of BK channel activation, we conclude that the BK channels underlying STOCs are exposed to a mean [Ca(2+)] on the order of 10 microM during a Ca(2+) spark. The membrane area over which a concentration > or =10 microM is reached has an estimated radius of 150-300 nm, corresponding to an area which is a fraction of one square micron. Moreover, given the constraints imposed by the estimated channel density and the Ca(2+) current during a spark, the BK channels do not appear to be uniformly distributed over the membrane but instead are found at higher density at the spark site.
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