ArticleThe British journal of ophthalmology2002
Polymerase chain reaction based detection of fungi in infected corneas.
Article in The British journal of ophthalmology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 88 citations in OpenAlex.
- Evaluation of Fungitell (1,3)-β-D-glucan assay in tear samples for rapid diagnosis of fungal keratitis.Journal of clinical microbiology · 2024Article
- [Corneal wound healing-Pathophysiology and principles].Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft · 2021Article
- Article
- An Omics Approach to Diagnosing or Investigating Fungal Keratitis.International journal of molecular sciences · 2019Review
- The pathogenic spectrum of fungal keratitis in northwestern China.International journal of ophthalmology · 2016Article
- Study of the prevalence and association of ocular chlamydial conjunctivitis in women with genital infection by Chlamydia trachomatis, Mycoplasma genitalium and Candida albicans attending outpatient clinic.International journal of ophthalmology · 2016Article
- Detection of fungi by conventional methods and semi-nested PCR in patients with presumed fungal keratitis.Current medical mycology · 2015Article
- Evaluation of molecular diagnosis in fungal keratitis. Ten years of experience.Journal of ophthalmic inflammation and infection · 2011Article
- Application of polymerase chain reaction (PCR) and PCR based restriction fragment length polymorphism for detection and identification of dermatophytes from dermatological specimens.Indian journal of dermatology · 2008Article
- Microbiological diagnosis of infective keratitis: comparative evaluation of direct microscopy and culture results.The British journal of ophthalmology · 2006Article
- Current perspectives on ophthalmic mycoses.Clinical microbiology reviews · 2003Review
- Development and Clinical Evaluation of a CRISPR/Cas12a-Based Nucleic Acid Detection Platform for the Diagnosis of Keratomycoses.Ophthalmology scienceArticle
- Emerging diagnostic modalities in microbial keratitis: Beyond culture and smear.Saudi journal of ophthalmology : official journal of the Saudi Ophthalmological SocietyArticle
- Investigative modalities in infectious keratitis.Indian journal of ophthalmologyArticle
- The persistent dilemma of microbial keratitis: Global burden, diagnosis, and antimicrobial resistance.Survey of ophthalmologyReview
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
aimsTo evaluate a polymerase chain reaction (PCR) based assay to detect fungi in scrapings from infected corneas.
methodsA PCR assay was developed to amplify a portion of the fungal 18S ribosome gene. Corneal scrapings from 30 patients with presumed infectious keratitis were evaluated using this assay, as well as by standard microbiological techniques, and the results were compared. Conjunctival swabs from each patient's healthy, fellow eye were also evaluated by PCR.
resultsPCR and fungal culture results matched (were both positive or both negative for fungi) in 22 (74%) of 30 scrapings from infected corneas. Three (10%) of 30 samples were PCR positive but fungal culture negative; two of these appeared clinically to represent fungal infections, and the third was clinically indeterminate. Four (13%) scrapings were positive by PCR but also by bacterial and not fungal culture. One specimen (3%) was PCR negative but fungal culture positive. Of the conjunctival swabs from each patient's healthy fellow eye, five (17%) of 30 were positive by PCR, and the opposite, infected eye of all five of these harboured a fungal infection.
conclusionsPCR is promising as a means to diagnose fungal keratitis and offers some advantages over culture methods, including rapid analysis and the ability to analyse specimens far from where they are collected.
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