ArticleThe Journal of cell biology2002
The roles of Fzy/Cdc20 and Fzr/Cdh1 in regulating the destruction of cyclin B in space and time.
Article in The Journal of cell biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers.
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Who cites it
69 citing papers in PubMed, 166 citations in OpenAlex.
- The genetics and evolution of moth melanism in the absence of strong natural selection.National science review · 2026Article
- USP8 and Hsp70 regulate endoreplication by synergistically promoting Fzr deubiquitination and stabilization.Science advances · 2025Article
- A force-sensitive mutation reveals a non-canonical role for dynein in anaphase progression.The Journal of cell biology · 2024Article
- Analysis of nondegradable cyclins reveals distinct roles of the mitotic cyclins in Drosophila meiosis.G3 (Bethesda, Md.) · 2024Article
- Two-fluid dynamics and micron-thin boundary layers shape cytoplasmic flows in earlyProceedings of the National Academy of Sciences of the United States of America · 2023Article
- APC/C-dependent degradation of Spd2 regulates centrosome asymmetry in Drosophila neural stem cells.EMBO reports · 2023Article
- Two-fluid dynamics and micron-thin boundary layers shape cytoplasmic flows in earlybioRxiv : the preprint server for biology · 2023Article
- Fzr regulates silk gland growth by promoting endoreplication and protein synthesis in the silkworm.PLoS genetics · 2023Article
- USP13 modulates the stability of the APC/C adaptor CDH1.Molecular biology reports · 2022Article
- The APC/C targets the Cep152-Cep63 complex at the centrosome to regulate mitotic spindle assembly.Journal of cell science · 2022Article
- Article
- The careful control of Polo kinase by APC/C-Ube2C ensures the intercellular transport of germline centrosomes duringOpen biology · 2021Article
- Cyclin B3 activates the Anaphase-Promoting Complex/Cyclosome in meiosis and mitosis.PLoS genetics · 2020Article
- A novel transcriptional cascade is involved in Fzr-mediated endoreplication.Nucleic acids research · 2020Article
- The Role of Hydrogen Peroxide and Peroxiredoxins throughout the Cell Cycle.Antioxidants (Basel, Switzerland) · 2020Review
- Coordination of Embryogenesis by the Centrosome in Drosophila melanogaster.Results and problems in cell differentiation · 2019Review
- Tellurium notebooks-An environment for reproducible dynamical modeling in systems biology.PLoS computational biology · 2018Article
- Plasmodium APC3 mediates chromosome condensation and cytokinesis during atypical mitosis in male gametogenesis.Scientific reports · 2018Article
- Degradation of FBXO31 by APC/C is regulated by AKT- and ATM-mediated phosphorylation.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- APC/CG3 (Bethesda, Md.) · 2017Article
9 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
In Drosophila cells cyclin B is normally degraded in two phases: (a) destruction of the spindle-associated cyclin B initiates at centrosomes and spreads to the spindle equator; and (b) any remaining cytoplasmic cyclin B is degraded slightly later in mitosis. We show that the APC/C regulators Fizzy (Fzy)/Cdc20 and Fzy-related (Fzr)/Cdh1 bind to microtubules in vitro and associate with spindles in vivo. Fzy/Cdc20 is concentrated at kinetochores and centrosomes early in mitosis, whereas Fzr/Cdh1 is concentrated at centrosomes throughout the cell cycle. In syncytial embryos, only Fzy/Cdc20 is present, and only the spindle-associated cyclin B is degraded at the end of mitosis. A destruction box-mutated form of cyclin B (cyclin B triple-point mutant [CBTPM]-GFP) that cannot be targeted for destruction by Fzy/Cdc20, is no longer degraded on spindles in syncytial embryos. However, CBTPM-GFP can be targeted for destruction by Fzr/Cdh1. In cellularized embryos, which normally express Fzr/Cdh1, CBTPM-GFP is degraded throughout the cell but with slowed kinetics. These findings suggest that Fzy/Cdc20 is responsible for catalyzing the first phase of cyclin B destruction that occurs on the mitotic spindle, whereas Fzr/Cdh1 is responsible for catalyzing the second phase of cyclin B destruction that occurs throughout the cell. These observations have important implications for the mechanisms of the spindle checkpoint.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.