ArticleJournal of bacteriology2002
RelA protein is involved in induction of genetic competence in certain Bacillus subtilis strains by moderating the level of intracellular GTP.
Article in Journal of bacteriology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 93 citations in OpenAlex.
- Ribosomal allostery as a potential regulator of bacterial dormancy.Nature communications · 2026Article
- Cell density and single-cell heterogeneity reveal distinct competence induction dynamics in the high-GC Gram-positive Micrococcus luteus.BMC microbiology · 2026Article
- A mutation in RNA polymerase imparts resistance to β-lactams by preventing dysregulation of amino acid and nucleotide metabolism.Cell reports · 2025Article
- Inactivation of guanylate kinase inHeliyon · 2024Article
- CcpA and CodY Regulate CRISPR-Cas System ofMicrobiology spectrum · 2023Article
- The molecular mechanism for carbon catabolite repression of the chitin response in Vibrio cholerae.PLoS genetics · 2023Article
- Soil Component: A Potential Factor Affecting the Occurrence and Spread of Antibiotic Resistance Genes.Antibiotics (Basel, Switzerland) · 2023Review
- Termination factor Rho mediates transcriptional reprogramming of Bacillus subtilis stationary phase.PLoS genetics · 2023Article
- Reconstitution of microtubule into GTP-responsive nanocapsules.Nature communications · 2022Article
- Stringent Response Regulates Stress Resistance in CyanobacteriumFrontiers in microbiology · 2020Article
- Amino acids as nutritional factors and (p)ppGpp as an alarmone of the stringent response regulate natural transformation in Micrococcus luteus.Scientific reports · 2019Article
- A Nutrient-Regulated Cyclic Diguanylate Phosphodiesterase Controls Clostridium difficile Biofilm and Toxin Production during Stationary Phase.Infection and immunity · 2017Article
- Insights into microbial cryptic gene activation and strain improvement: principle, application and technical aspects.The Journal of antibiotics · 2017Review
- The Borrelia burgdorferi RelA/SpoT Homolog and Stringent Response Regulate Survival in the Tick Vector and Global Gene Expression during Starvation.PLoS pathogens · 2015Article
- ComGA-RelA interaction and persistence in the Bacillus subtilis K-state.Molecular microbiology · 2015Article
- Many means to a common end: the intricacies of (p)ppGpp metabolism and its control of bacterial homeostasis.Journal of bacteriology · 2015Review
- RelA inhibits Bacillus subtilis motility and chaining.Journal of bacteriology · 2015Article
- The mthA mutation conferring low-level resistance to streptomycin enhances antibiotic production in Bacillus subtilis by increasing the S-adenosylmethionine pool size.Journal of bacteriology · 2014Article
- CodY-mediated regulation of the Staphylococcus aureus Agr system integrates nutritional and population density signals.Journal of bacteriology · 2014Article
- The stringent response of Staphylococcus aureus and its impact on survival after phagocytosis through the induction of intracellular PSMs expression.PLoS pathogens · 2012Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We found that the ability to develop genetic competence of a certain relaxed (relA) aspartate-auxotrophic strain of Bacillus subtilis is significantly lower than that of the isogenic stringent (relA+) strain. Transcriptional fusion analysis utilizing a lacZ reporter gene indicated that the amount of the ComK protein, known as the key protein for competence development, is greatly reduced in the relaxed strain than in the stringent strain. We also found that the addition of decoyinine, a GMP synthetase inhibitor, induces expression of a competence gene (comG) in the relaxed strain, accompanied by a pronounced decrease in the level of intracellular GTP as measured by high-performance liquid chromatography. The transformation efficiency of the relaxed strain increased 100-fold when decoyinine was added at t0 (the transition point between exponential to stationary growth phase). Conversely, supplementation of guanosine together with decoyinine completely abolished the observed effect of adding decoyinine on competence development. Furthermore, the impaired ability of the relaxed strain for competence development was completely restored by disrupting the codY gene, which is known to negatively control comK expression. Our results indicate that the RelA protein plays an essential role in the induction of competence development at least under certain physiological conditions by reducing the level of intracellular GTP and overcoming CodY-mediated regulation.
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