ArticleJournal of bacteriology2002
Network of hydrogenase maturation in Escherichia coli: role of accessory proteins HypA and HybF.
Article in Journal of bacteriology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 106 citations in OpenAlex.
- Genome-wide analysis of the Escherichia coli Keio collection reveals genetic determinants associated with ferulic acid responses.Folia microbiologica · 2026Article
- Structural investigations connect the disordered N-terminal extension of HypB to the activities of HypB and SlyD in E. coli.Protein science : a publication of the Protein Society · 2025Article
- Article
- Molecular Hydrogen Metabolism: a Widespread Trait of Pathogenic Bacteria and Protists.Microbiology and molecular biology reviews : MMBR · 2020Review
- Bimodal Nickel-Binding Site onInorganic chemistry · 2019Article
- A whole-cell, high-throughput hydrogenase assay to identify factors that modulate [NiFe]-hydrogenase activity.The Journal of biological chemistry · 2019Article
- Nickel Metalloregulators and Chaperones.Inorganics · 2019Article
- Maturation of the [Ni-4Fe-4S] active site of carbon monoxide dehydrogenases.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2018Review
- Co(II) and Ni(II) binding of theThe Journal of biological chemistry · 2018Article
- Proteolytic cleavage orchestrates cofactor insertion and protein assembly in [NiFe]-hydrogenase biosynthesis.The Journal of biological chemistry · 2017Article
- Anaerobic Formate and Hydrogen Metabolism.EcoSal Plus · 2016Review
- Genome annotation provides insight into carbon monoxide and hydrogen metabolism in Rubrivivax gelatinosus.PloS one · 2014Article
- Metal transfer within the Escherichia coli HypB-HypA complex of hydrogenase accessory proteins.Biochemistry · 2013Article
- Specific metal recognition in nickel trafficking.Biochemistry · 2012Review
- Structural basis for GTP-dependent dimerization of hydrogenase maturation factor HypB.PloS one · 2012Article
- Interaction between hydrogenase maturation factors HypA and HypB is required for [NiFe]-hydrogenase maturation.PloS one · 2012Article
- Probing the origin of the metabolic precursor of the CO ligand in the catalytic center of [NiFe] hydrogenase.The Journal of biological chemistry · 2011Article
- Protein interactions and localization of the Escherichia coli accessory protein HypA during nickel insertion to [NiFe] hydrogenase.The Journal of biological chemistry · 2011Article
- Genome data mining and soil survey for the novel group 5 [NiFe]-hydrogenase to explore the diversity and ecological importance of presumptive high-affinity H(2)-oxidizing bacteria.Applied and environmental microbiology · 2011Article
- Relationship between the GTPase, metal-binding, and dimerization activities of E. coli HypB.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2011Article
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3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have studied the roles of the auxiliary protein HypA and of its homolog HybF in hydrogenase maturation. A mutation in hypA leads to the nearly complete blockade of maturation solely of hydrogenase 3 whereas a lesion in hybF drastically but not totally reduces maturation and activity of isoenzymes 1 and 2. The residual level of matured enzymes in the hybF mutant was shown to be due to the function of HypA; HybF, conversely, was responsible for a minimal residual activity of hydrogenase 3 in the mutant hypA strain. Accordingly, a hypA DeltahybF double mutant was completely blocked in the maturation process. However, the inclusion of high nickel concentrations in the medium could restore limited activity of all three hydrogenases. The results of this study and of previous work (M. Blokesch, A. Magalon, and A. Böck, J. Bacteriol. 189:2817-2822, 2001) show that the maturation of the three functional hydrogenases from Escherichia coli is intimately connected via the activity of proteins HypA and HypC and of their homologs HybF and HybG, respectively. The results also support the suggestion of Olson et al. (J. W. Olson, N. S. Mehta, and R. J. Maier, Mol. Microbiol. 39:176-182, 2001) that HypA cooperates with HypB in the insertion of nickel into the precursor of the large hydrogenase subunit. Whereas HypA is predominantly involved in the maturation of hydrogenase 3, HybF takes over its function in the maturation of isoenzymes 1 and 2.
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